肥胖小鼠血清铁调素水平变化及其与瘦素的关系-东南大学学报医学版

肥胖小鼠血清铁调素水平变化及其与瘦素的关系

单位：1. 首都医科大学公共卫生学院, 北京 100069;
2. 首都医科大学实验动物科学部, 北京 100069

分类号：R589.2

出版年·卷·期（页码）：2014·33·第六期（703-706）

摘要：

目的: 研究饮食诱导肥胖小鼠血清铁调素水平的变化及其与瘦素的相关性.方法: C57BL/6J小鼠随机分为正常对照组和肥胖组,每组6只.对照组饲以普通饲料,肥胖组以高脂饲料喂养12周.建模成功后小鼠心脏取血分离血清,用ELISA试剂盒测定血清中瘦素、铁调素的含量.结果: 肥胖小鼠血清瘦素、铁调素含量显著高于正常对照小鼠[(33.92±9.39)μg·L^-1 vs (15.32±5.48)μg·L^-1,P<0.01;(22.51±7.53)μg·L^-1 vs (14.03±1.08)μg·L^-1,P<0.05];相关分析表明,血清铁调素与瘦素水平间呈显著正相关关系(r=0.917,P<0.05).结论: 肥胖小鼠血清铁调素水平升高,并且铁调素水平与瘦素浓度正相关.

Objective: To study the change in the concentration of hepcidin and its correlation with leptin in obese mice. Methods: C57BL/6J mice were randomly divided into two groups with 6 in each.Mice in the control group were put on a normal diet, and the obese group were fed with a high-fat diet to establish obese mice model. After 12 weeks of interventions,mice were sacrificed and blood samples were collected. Serum leptin and hepcidin contents were detected using enzyme-linked immunosorbent assay. Results: Compared with the control group,serum leptin level in the obese model group was significantly higher[(33.92±9.39)μg·L^-1 vs (15.32±5.48)μg·L^-1,P<0.01],serum hepcidin level in the model group increased significantly[(22.51±7.53)μg·L^-1 vs (14.03±1.08)μg·L^-1,P<0.05] as well. Analysis suggested positive correlation between hepcidin and leptin levels(r=0.917,P<0.05). Conclusion: The concentrations of leptin and hepcidin in the serum of obese mice are significantly higher than in normal mice, and a positive correlation exists between leptin and hepcidin levels.

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