羟基磷灰石纳米管于大鼠股骨肌袋异位成骨的实验研究-东南大学学报医学版

羟基磷灰石纳米管于大鼠股骨肌袋异位成骨的实验研究

作者：王晓波¹ 陈一心² 郑欣² 郭向可³ 王骏飞² 李东亚² 邱旭升² 施鸿飞² 王杨雨凡⁴

单位：1. 东南大学鼓楼临床医学院骨科, 江苏南京 210008;
2. 南京大学医学院附属鼓楼医院骨科, 江苏南京 210008;
3. 南京大学化学化工学院, 江苏南京 210008;
4. 徐州市中心医院骨科, 江苏徐州 221000

关键词：羟基磷灰石纳米管大鼠骨修复异位成骨

分类号：R-332

出版年·卷·期（页码）：2014·33·第六期（699-702）

摘要：

目的: 用动物体内实验的方法验证羟基磷灰石(HAP)纳米管在大鼠股骨肌袋内的异位诱导成骨能力.方法: 选用10只雄性大鼠,双侧手术共20侧,采用自身对照的方法,在全麻下大鼠右侧股骨肌袋内植入HAP纳米管粉末约2 g,左侧肌袋打开后缝合作为对照,分别在术后当日及4、8、12周行X线及CT扫描检查,第12周即将实验动物处死取植入物部位进行组织病理学检查.结果: 术后所有大鼠均未发生感染、死亡等并发症;术后各期植入区X线及CT扫描均可见HAP纳米管粉末,未见明显与骨组织密度相当的高密度影;病理学检查植入物周围未见软骨细胞成骨、毛细血管生成及HAP纳米管粉末降解.结论: 单纯体外应用HAP纳米管无明显异位诱导成骨的作用,其异位成骨能力有待进一步实验验证.

Objective: To investigate the ectopic bone formation ability of hydroxyapatite nanotubes after implanted into femoral muscle pouch in rats. Methods: The hydroxyapatite nanotubes were implanted into the left femoral muscle pouch of ten rats.Bilateral operation was performed for self-control and the right femoral muscle pouch of ten rats were just open but implant nothing.After surgery, X-rays and CT were taken immediately after surgery,4-week,8-week, 12-week postoperatively.The rats were sacrificed twelve weeks after surgery and the specimens were harvested for pathologic analysis. Results: No rats had sign of postoperative infection; In each observation period,the hydroxyapatite nanotubes could be detected but the very high density of bone could not be seen;the bone cartilage cells,capillary formation and degradation of hydroxyapatite nanotubes powder could not be found around the implant. Conclusion: The ectopic formation ability of pure hydroxyapatite nanotubes is limited and need to be confirmed in further study.

参考文献：

[1] BANWART J C,ASHER M A,HASSANEIN R S.Iliac crest bone graft harvest donor site morbidity.A statistical evaluation[J].Spine,1995,20(9): 1055-1060.
[2] SINGH H,LEVI A D.Bone graft and bone substitute biology[M].Berlin: Springer,2014: 147-152.
[3] LIANGA G,YANGB Y,OHB S,et al.Ectopic osteoinduction and early degradation of rhBMP2—loaded porous β-tricalcium phosphatein mice[J].Biomaterials,2005,26(20): 4265-4271.
[4] KUBLER N R.Osteoinduktion und-reparation[M].Berlin: Springer,1997: 2-25.
[5] GUO X K,YU L,CHEN L,et al.Organoamine-assisted biomimetic synthesis of faceted hexagonal hydroxyapatite nanotubes with prominent stimulation activity for osteoblast proliferation[J].J Mater Chem,2014,2(13): 1760-1763.
[6] 徐靖宏,王炜.骨骼肌异位成骨载体材料的研究进展[J].口腔材料器械杂志,2003,12(2): 97-99.
[7] WEBSTER T J,ERGUN C,DOREMUS R H,et al.Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics[J].J Biomed Mater Res,2000,51(3): 475-483.
[8] 左奕,李玉宝.纳米骨修复生物材料研究进展[J].东南大学学报: 医学版,2011,30(1): 82-91.
[9] BRUDER B S,KRAUS K H,GOLDBERG V M,et al.The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects[J].JBJS,1998,80A(7): 985-996.
[10] SCOTT M A,LEVI B,ASKARINAM A,et al.Brief review of models of ectopic bone formation[J].Stem Cells Dev,2012,21(5): 655-667.
[11] HELIOTIS M,LAVERY K,RIPAMONTI U,et al.Transformation of a prefabricated hydroxyapatite/osteogenic protein-1 implant into a vascularised pedicled bone flap in the human chest[J].Int J Oral Maxillofac Surg,2006,35(3): 265-269.
[12] YANG Z,YUAN H,TONG W,et al.Osteogenesis in extraskeletally implanted porous calcium phosphate ceramics: variability among different kinds of animals[J].Biomaterials,1996,17(22): 2131-2137.
[13] RIPAMONTI U.Osteoinduction in Dorous hydroxyapatite imilanted inhete rotopic sites of different animal models[J].Biomaterials,1996,17(1): 31-35.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录