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羟基磷灰石纳米管于大鼠股骨肌袋异位成骨的实验研究
作者:王晓波1  陈一心2  郑欣2  郭向可3  王骏飞2  李东亚2  邱旭升2  施鸿飞2  王杨雨凡4 
单位:1. 东南大学鼓楼临床医学院 骨科, 江苏 南京 210008;
2. 南京大学医学院附属鼓楼医院 骨科, 江苏 南京 210008;
3. 南京大学 化学化工学院, 江苏 南京 210008;
4. 徐州市中心医院 骨科, 江苏 徐州 221000
关键词:羟基磷灰石纳米管 大鼠 骨修复 异位成骨 
分类号:R-332
出版年·卷·期(页码):2014·33·第六期(699-702)
摘要:

目的: 用动物体内实验的方法验证羟基磷灰石(HAP)纳米管在大鼠股骨肌袋内的异位诱导成骨能力.方法: 选用10只雄性大鼠,双侧手术共20侧,采用自身对照的方法,在全麻下大鼠右侧股骨肌袋内植入HAP纳米管粉末约2 g,左侧肌袋打开后缝合作为对照,分别在术后当日及4、8、12周行X线及CT扫描检查,第12周即将实验动物处死取植入物部位进行组织病理学检查.结果: 术后所有大鼠均未发生感染、死亡等并发症;术后各期植入区X线及CT扫描均可见HAP纳米管粉末,未见明显与骨组织密度相当的高密度影;病理学检查植入物周围未见软骨细胞成骨、毛细血管生成及HAP纳米管粉末降解.结论: 单纯体外应用HAP纳米管无明显异位诱导成骨的作用,其异位成骨能力有待进一步实验验证.

Objective: To investigate the ectopic bone formation ability of hydroxyapatite nanotubes after implanted into femoral muscle pouch in rats. Methods: The hydroxyapatite nanotubes were implanted into the left femoral muscle pouch of ten rats.Bilateral operation was performed for self-control and the right femoral muscle pouch of ten rats were just open but implant nothing.After surgery, X-rays and CT were taken immediately after surgery,4-week,8-week, 12-week postoperatively.The rats were sacrificed twelve weeks after surgery and the specimens were harvested for pathologic analysis. Results: No rats had sign of postoperative infection; In each observation period,the hydroxyapatite nanotubes could be detected but the very high density of bone could not be seen;the bone cartilage cells,capillary formation and degradation of hydroxyapatite nanotubes powder could not be found around the implant. Conclusion: The ectopic formation ability of pure hydroxyapatite nanotubes is limited and need to be confirmed in further study.

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