ApoAI对原代培养人Müller细胞生长的影响-东南大学学报医学版

ApoAI对原代培养人Müller细胞生长的影响

单位：苏州大学附属第三医院眼科, 江苏常州 213000

关键词：糖尿病视网膜病变增殖性糖尿病视网膜病变视网膜Mü ller细胞载脂蛋白AI 流式细胞术

分类号：R-33;R587.2

出版年·卷·期（页码）：2014·33·第五期（609-612）

摘要：

目的：研究ApoAI对原代培养人Müller细胞生长的影响。方法：原代培养人的视网膜Müller细胞后用不同浓度的ApoAI刺激Müller细胞24h，采用流式细胞术检测ApoAI作用24h后Müller细胞的细胞周期，比较各组细胞的细胞周期；采用流式细胞术和荧光照相法检测各组Müller细胞细胞凋亡水平，采用单因素方差分析方法比较各组间不同细胞周期间不同细胞数的变化以及各组细胞的凋亡数。结果与结论：流式细胞术结果显示，ApoAI可以抑制人Müller细胞的增殖，且随ApoAI浓度的增加抑制作用增强（P<0.05）；ApoAI对细胞的凋亡无明显作用（P>0.05）。

Objective: To investigate effects of ApoAI on the growth of cultured human retinal Müller cells. Methods: In vitro,human retinal Müller cells were successfully cultured from the remaining part of the eye after corneal transplantation. With different concentrations of ApoAI stimulated, the cycles and the levels of apoptosis of Müller cells were detected by Flow Cytometry(FCM) after 24h,also,we took the photofluorography of the apoptotic cells.Then the results were analyzed with one-way anova.Results and conclusion: The growth of cultured retinal Müller cells were significantly restrained by ApoAI and this effect was concentration dependent in a certain range(P<0.05); ApoAI had no significant effect on apoptosis(P>0.05).

参考文献：

[1] SAITO H,DHANASEKARAN P,NGUYEN D,et al.Alpha-helix formation is required for high affinity binding of human apolipoprotein A-I to lipids[J].The Journal of Biological Chemistry,2004,279(20):20974-20981.
[2] 张扬,冉建民,徐刚.2型糖尿病患者高密度脂蛋白水平变化分析[J].中国基层医药,2007,14(7):1196-1197.
[3] 王慧,王晓琴.糖尿病合并急性脑梗死老年患者的血脂分析[J].实用医学杂志,2011,28(3):193-195.
[4] AYYOBI A F,BRUNZELL J D.Lipoprotein distribution in the metabolic syndrome,type 2 diabetes mellitus,and familial combined hyperlipidemia[J].The American Journal of Cardiology,2003,92(4A):27J-33J.
[5] 朱茂丽,刘瑶,陈平圣,等.糖基化终产物对视网膜Mailer细胞表达血管内皮生长因子的影响[J].中国糖尿病杂志,2008,16(11):691-693.
[6] GUIDRY C,KING J L,MASON J O.Fibrocontractive Muller cell phenotypes in proliferative diabetic retinopathy[J].Investigative Ophthalmology & Visual Science,2009,50(4):1929-1939.
[7] NEWMAN E,REICHENBACH A.The Muller cell:a functional element of the retina[J].Trends in Neurosciences,1996,19(8):307-312.
[8] 杨开燕,钟栩,卢玉俊.血浆载脂蛋白的研究进展[J].临床荟萃,2012,27(14):1268-1271.
[9] 何爱琴,吴小梅,缪珩.2型糖尿病患者ApoAl/ApoB之值与其血管并发症关系分析[J].现代医学,2012,40(5):556-559.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录