新型荧光探针应用于帕金森病模型中NO的检测-东南大学学报医学版

新型荧光探针应用于帕金森病模型中NO的检测

单位：南京大学生命科学学院医药生物技术国家重点实验室, 江苏南京 210093

分类号：R-332;R742.5

出版年·卷·期（页码）：2014·33·第五期（555-560）

摘要：

目的：研究帕金森病中一氧化氮（NO）检测的新方法。方法：建立帕金森病的细胞与动物模型，检测小鼠相关行为学与生化指标的改变并通过新型探针MNIP-Cu荧光成像的方法检测NO浓度的变化。结果：帕金森病的细胞与动物模型中荧光探针MNIP-Cu检测到NO浓度显著升高，同时小鼠运动能力受到损伤、多巴胺合成减少；当NO合成受到抑制后，小鼠的运动能力和多巴胺的合成均显著恢复。荧光探针MNIP-Cu能够成功检测NO含量的变化。结论：新型荧光探针MNIP-Cu实现了对帕金森病体内外模型中NO的直接检测，为阐明NO在帕金森发病机制中的作用提供了新的研究手段。

Objective: To develop a new method of nitric oxide detection in Parkinson disease.Methods: Using a Parkinson disease model in vitro and in vivo,we tested the behavior and biochemical changes of rat, and then detected the change of nitric oxide concentration through a novel fluorescent probe of MNIP-Cu.Results:The movement ability of rat was damaged with reduced dopamine synthesis in the Parkinson disease model.Consistently, the NO level was detected successfully by the MNIP-Cu fluorescence probe with significant increase. On the other hand, the rat's exercise capacity and dopamine production level was significantly restored after the inhibition of NO synthesis. Taking together, the NO production level can be detected successfully by the fluorescence probe of MNIP-Cu. Conclusion:We detect NO directly in Parkinson disease model in vitro and in vivo through novel fluorescent probe, which provides a new way to study the role of NO in the pathogenesis of the Parkinson disease.

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