IL-6对小鼠肾上腺皮质细胞系Y1合成皮质醇的影响-东南大学学报医学版

IL-6对小鼠肾上腺皮质细胞系Y1合成皮质醇的影响

单位：1. 东南大学医学院, 江苏南京 210009;
2. 东南大学附属第二医院, 江苏南京 210003

分类号：R586

出版年·卷·期（页码）：2014·33·第四期（456-460）

摘要：

目的：探讨白介素6（IL-6）对小鼠肾上腺皮质细胞系Y1合成皮质醇能力及合成过程中类固醇急性调节蛋白（StAR）相关基因表达的影响。方法：100 ng·ml^-1 IL-6单独或联合10^-9 mol·L^-1促肾上腺皮质激素（ACTH）处理Y1细胞后，台盼蓝拒染法检测细胞活力，放免法检测细胞皮质醇合成量，实时荧光定量PCR法检测StAR mRNA表达水平。结果：IL-6单独或联合ACTH处理Y1细胞后，皮质醇合成量增加（P<0.05），同时StAR mRNA表达水平上升（P<0.05）。结论：IL-6可单独或联合ACTH通过促进StAR mRNA表达增强Y1细胞合成皮质醇的能力。

Objective: To explore the effects of interleukin-6(IL-6) on synthesis of corticosteroid in mouse adrenal cortex cell lines Y1(Y1cells) and the steroid acute regulatory protein(StAR) related gene.Methods: The Y1 cells were treated with 100 ng·ml^-1 IL-6 alone or combined with 10^-9 mol·L^-1 adrenocorticotropic hormone(ACTH).The cell viability was detected by blue dye exclusion method.Corticosteroid synthesis level of the cells was determined by radioimmunoassay.The change of StAR mRNA was tested by real-time PCR.Results: After treated with IL-6 or/and ACTH,synthesis of corticosteroid had been increased and StRA mRNA expression was significantly increased(P<0.05).Conclusion: IL-6 alone or combined with ACTH could promote the synthesis of corticosteroid by increasing StAR mRNA expression.

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