孕鼠暴露低剂量邻苯二甲酸二己酯对仔鼠脂肪分布的影响-东南大学学报医学版

孕鼠暴露低剂量邻苯二甲酸二己酯对仔鼠脂肪分布的影响

单位：1. 中国医科大学公共卫生学院营养与食品卫生学教研室,辽宁沈阳 110001;
2. 中国医科大学附属盛京医院骨外科,辽宁沈阳 110004

分类号：R589.2;R-33

出版年·卷·期（页码）：2014·33·第二期（147-150）

摘要：

目的：探讨孕鼠暴露低剂量邻苯二甲酸二己酯（di-2-ethylhexylphthalate，DEHP）对仔鼠脂肪分布的影响及机制。方法：将C57BL/6J孕鼠分为2组，对照组喂饲基础饲料，DEHP组喂饲添加质量分数为0.06% DEHP的基础饲料，至仔鼠出生。9周后，处死仔鼠，切取附睾周围和腹股沟皮下脂肪组织，称质量；实时定量RT-PCR法检测Gpc4、Tbx15 mRNA表达。结果：DEHP组仔鼠附睾脂肪组织质量显著高于对照组（P<0.05）；对照组和DEHP组仔鼠附睾脂肪组织Gpc4 mRNA表达均显著高于皮下脂肪组织（P<0.05），而Tbx15 mRNA表达则显著低于皮下脂肪组织（P<0.01）；相比对照组，DEHP组仔鼠附睾脂肪组织Gpc4 mRNA及皮下脂肪组织Tbx15 mRNA表达均显著增加（P<0.01）。结论：胚胎期低剂量DEHP暴露可能通过改变内脏及皮下Gpc4和Tbx15的表达，影响脂肪组织的分布。

Objective: To investigate the effect and mechanism of low dose di-2-ethylhexylphthalate (DEHP) exposure on fat distribution of offspring in pregnant mice. Methods: Pregnant C57BL/6J mice were divided into two groups. Mice in control group were fed with chow diet, and mice in DEHP group were fed with chow diet supplemented with 0.06%(w/w)DEHP till their offspring was born. Nine weeks after birth, the mice were sacrificed. Epididymal and inguinal subcutaneous adipose tissues were dissected and weighted. The expression of Gpc4 and Tbx15 mRNA was measured by real-time reverse transcription polymerase chain reaction (RT-PCR) method. Results: The weight of epididymal adipose tissues in DEHP group was significantly higher than that in control group (P<0.05). The expression of Gpc4 mRNA in epididymal adipose tissues was significantly higher than that in subcutaneous adipose tissues (P<0.05), while the expression of Tbx15 mRNA was significantly lower than that in subcutaneous adipose tissues (P<0.01) in both control and DEHP group. Compared with control group, Gpc4 mRNA expression in epididymal adipose tissues and Tbx15 mRNA expression in subcutaneous adipose tissues were increased (P<0.01). Conclusion: Low dose DEHP exposure in mice prenatal period perhaps affects fat distribution via modifying the expression of Gpc4 and Tbx15 mRNA in visceral and subcutaneous adipose tissues.

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