玉屏风散对Hepa1-6肝癌荷瘤小鼠免疫调节的影响-东南大学学报医学版

玉屏风散对Hepa1-6肝癌荷瘤小鼠免疫调节的影响

单位：苏州市中医医院中医药研究所, 江苏苏州 215009

分类号：R735.7;R73-361

出版年·卷·期（页码）：2014·33·第一期（34-39）

摘要：

目的：探讨玉屏风散对肝癌荷瘤小鼠机体免疫的调节作用。方法：采用皮下接种Hepa1-6肿瘤细胞建立肝癌荷瘤C57BL/6小鼠模型；应用流式细胞技术检测和分析荧光标记的免疫细胞亚群。结果：玉屏风散20、25、30 g生药·kg^-1对Hepa1-6肝癌荷瘤C57小鼠的抑瘤率分别为18.86%、28.81%、37.44%。与模型对照组比较，玉屏风散组胸腺重量和胸腺指数无变化，脾重和脾脏指数均呈上升趋势，脾脏中CD19⁺B细胞数量、CD19⁺CD80⁺B细胞比例、巨噬细胞比例和数量、CD4⁺T和CD8⁺T细胞数量以及肿瘤组织中CD4⁺T和CD8⁺T细胞比例均显著增加（P<0.05，P<0.01），而脾脏中CD19⁺B细胞、CD4⁺T和CD8⁺T细胞的比例均无变化（P >0.05）。结论：玉屏风散对肝癌荷瘤小鼠机体免疫具有广泛调节和增强作用，为玉屏风散在临床抗肝癌中的合理应用提供了实验依据。

Objective: To research the immunoregulation effects of Yupingfeng Decoction(YPF) towards the C57 mice injected mouse hepatoma carcinoma Hepa1-6 cells. Methods: Hepatoma carcinoma model was established in C57BL/6 mice by subcutaneously injection of Hepa1-6 tumor cells. The lymphocyte subsets of fluorescence labeled were detected and analyzed by flow cytometry. Results: The anti-tumor rates of YPF-treated mice with 20,25,30 g·kg^-1 were 18.86%,28.81% and 37.44% respectively.Compared with control model group, the YPF-treated groups had rising trend with spleen weight and coefficient, and no change on thymus weight and coefficient. And the YPF-treated groups had significant increased of spleen CD19⁺ B cells number, spleen CD19⁺CD80⁺ B cells proportion, spleen macrophage cells number and proportion, spleen CD4⁺ and CD8⁺ T cells number, and the proportion of CD4⁺ and CD8⁺ T cells in neoplastic tissues(P<0.05, P<0.01), but the spleen CD19⁺ B and CD4⁺ T and CD8⁺ T cells proportion had no difference(P >0.05).Conclusion:YPF has a extensive regulation and enhancement effect on immune system of tumor bearing mice,which provided the experimental basis for the reasonable application of YPF in clinical against hepatocellular carcinoma.

参考文献：

[1] 杜鹃.中药抗肿瘤免疫的进展[J].中医药临床杂志, 2004, 16(5):507-509.
[2] 杜建.中药抗肿瘤机制研究进展[J].福建中医学院学报, 2009, 19(4):1-5.
[3] 林琳, 沈洪, 王立新, 等.黄芪甲苷、β-榄香烯对小鼠树突状细胞免疫功能的影响[J].东南大学学报:医学版, 2011, 30(2):294-297.
[4] MENG M B, CUI Y L, GUAN Y S, et al.Traditional Chinese medicine plus transcatheter arterial chemoembolization for unresectable hepatocellular carcinoma[J].J Altern Complement Med, 2008, 14(8):1027-1042.
[5] 周若梅, 李来秀.玉屏风散口服液的药理学研究[J].陕西中医, 2004, 25(1):71-73.
[6] 周春权, 姚欣, 林静瑜, 等.玉屏风散粗多糖的提取及药理实验[J].海峡药学, 2004, 16(2):38-40.
[7] 张红军, 鞠宝玲, 胡艳君, 等.玉屏风散对荷瘤小鼠肿瘤生长及IL-2与IL-2R基因表达的影响[J].医学信息, 2008, 21(5):656-659.
[8] 张红军, 唐小云, 鞠宝玲, 等.玉屏风散对S180荷瘤小鼠肿瘤生长及免疫功能的影响[J].细胞与分子免疫学杂志, 2008, 24(7):683-685.
[9] 陈奇.中药药理研究方法学[M].北京:人民卫生出版社, 1993:712-713.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录