清开灵对癫痫大鼠EAAs、IAAs干预作用的研究-东南大学学报医学版

清开灵对癫痫大鼠EAAs、IAAs干预作用的研究

单位：1.东南大学临床医学院儿科学系,江苏南京,210009； 2.东南大学附属中大医院儿科,江苏南京,210009

分类号：R285.5, R742.1

出版年·卷·期（页码）：2003·22·第六期（380-383）

摘要：

目的与方法:通过观测、对比使用清开灵注射液或苯巴比妥前后癫痫点燃模型大鼠海马组织谷氨酸(Glu)、γ-氨基丁酸(GABA)的含量以及神经元形态学的改变,以探索癫痫的发病机制以及清开灵注射液对癫痫的干预作用.结果:所有大鼠均达到癫痫模型点燃标准.使用清开灵的中药干预组与点燃模型组比较,Glu含量明显下降,GABA含量则明显升高(P<0.01),同时神经元形态学改变明显好转.结论:清开灵注射液可能具有减轻痫性发作,使神经元得到保护的作用.

Objective To discuss mechanisms of amino acid neurotransmitter in formation and developing process of epilepsy, and find out a new pathway of epileptic therapeutics.Methods 40 rats were evenly devided into 4 groups: kindled group,Qingkailing group,phenobarbital group and blank control group. Pentetrazole was injected into abdominal cavity to establish epileptic rat models. HPLC was used to detect the content of glutamate(Glu)and γ-aminobutyric acid(GABA)in epileptic rats, and followed by the comparison of Qingkailing parenteral solution and barbenyl intervention. Results All rats reached kindled standard. Then Glu content of Qingkailing group was lower than that of kindled model group, while GABA was higher(P<0.01). Conclusion Qingkailing parenteral solution seems to resolves epilepsy and protect neurons.

参考文献：

[1] Meldrum B S, AKBAR M T, CHAPMAN A G. Glutamate receptors and transporters in genetic and acquired models of epilepsy. 1999(2-3)
[2] Diehl R G, SMIALOWSKI A, GOTWO T. Development and persistence of kindled seizures after repeated injection of pentylenetetrazol in rats and guinea pigs. 1984(4). doi:10.1111/j.1528-1157.1984.tb03452.x
[3] Schreiber S S, BAUDRY M. Selective neuronal vulnerability in the hippocampus:a role for gene expression. 1995(4). doi:10.1016/0166-2236(95)94495-Q
[4] Allen I C, GRIEVE A, GRIFTITHS R. Differential changes in the content of amino acid neurotransmitters in discrete regions of the rat brain prior to the onset and during the course of homocysteine-induced seizures. 1986(22). doi:10.1111/j.1471-4159.1986.tb01780.x
[5] Charriaut Marlangue C, AGGOUN ZOUAOUI D, REPRESA A. Apoptotic features of selective neuronal death in ischemia,epilepsy and gp 120 toxicity. 1996(3). doi:10.1016/S0166-2236(96)80039-7
[6] HOUSER C R, ESCLAPEZ M. Vulnerability and plasticity of the GABA system in the pilocarpine model of spontaneous recurrent seizures. 1996(1). doi:10.1016/S0920-1211(96)00054-X
[7] ROWLEY H L, MARTIN K F, MARSDEN C A. Decreased GABA release following tonic-clonic seizures is associated with an increase in extracellular glutamate in rat hippocampus in vivo. 1995(2). doi:10.1016/0306-4522(95)00159-G

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录