人胃癌组织中环氧化酶-2mRNA的表达-东南大学学报医学版

人胃癌组织中环氧化酶-2mRNA的表达

单位：1.南京大学医学院附属鼓楼医院消化内科,江苏南京,210008； 2.东南大学附属中大医院消化内科,江苏南京,210009

分类号：R735.2

出版年·卷·期（页码）：2003·22·第六期（372-375）

摘要：

目的:检测环氧化酶-2(COX-2)mRNA在人胃癌、癌旁及正常胃黏膜组织中的表达情况,探讨其表达与胃癌临床病理特征的关系.方法:应用逆转录-聚合酶链反应(RT-PCR)技术检测24例胃癌、癌旁及正常胃黏膜组织中COX-2mRNA表达水平.结果:COX-2mRNA在胃癌组织中的表达水平为1.208±0.412,高于其在癌旁组织中的0.504±0.107和正常胃黏膜组织中的0.473±0.098,差异具有显著性(P<0.01);COX-2mRNA表达与患者性别、年龄及肿瘤大小、Lauren分型、分化程度均无显著性相关(P>0.05),而与肿瘤淋巴结转移、远处转移、临床分期和部位密切相关(P<0.01或P<0.05).结论:COX-2mRNA表达水平在人胃癌组织中增高,并与胃癌淋巴结转移、远处转移、分期、部位等临床病理特征密切相关,COX-2mRNA表达在人胃癌发生、发展、转移过程中发挥重要作用.

Objective To test the expression of COX-2 gene in human gastric carcinoma, adjacent nontumorous tissues and normal gastric mucosa. Methods Samples of cancer tissues, adjacent nontumorous tissues and normal gastric mucosa from twenty-four patients with primary gastric cancer were obtained during surgery. COX-2 mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR). Results The level of COX-2 mRNA in gastric carcinoma was significantly higher than that in adjacent nontumorous tissues and normal gastric mucosa (P< 0.01). There was no significant difference of the level of COX-2 mRNA between adjacent nontumorous tissues and normal gastric mucosa (P> 0.05). The level of COX-2 mRNA was not related to gender ( P> 0.05), age ( P> 0.05), tumor size ( P> 0.05), Lauren’s classification ( P> 0.05) and differentiation degree ( P> 0.05), but was correlated with lymphnode metastasis ( P< 0.01), distant metastasis ( P< 0.01), clinical stage ( P< 0.05) and tumor location ( P< 0.05). Conclusion The expression of COX-2 gene is increased in gastric carcinoma, and correlates with tumor lymphnode metastasis, distant metastasis, clinical stage and tumor location. COX-2 plays an important role in the growth and development of gastric carcinoma.

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