AGEs-BSA 对人肾系膜细胞分泌RANTES的影响初探-东南大学学报医学版

AGEs-BSA 对人肾系膜细胞分泌RANTES的影响初探

单位：东南大学,附属中大医院,江苏南京,210009

分类号：R334.1, R512.1

出版年·卷·期（页码）：2003·22·第六期（363-365）

摘要：

目的:探讨糖基化终产物(AGEs)对人肾系膜细胞(HRMC)分泌正常T细胞表达的调节活化蛋白(RANTES)的影响.方法:常规制备糖基化终产物-牛血清白蛋白(AGEs-BSA),干预体外培养的HRMC,用ELISA法检测培养上清RANTES水平.结果:AGEs-BSA(640mg*L-1) 干预HRMC后8h,培养液中RANTES水平开始逐步升高,于48h达峰值,对照组BSA则未见升高(P<0.01).结论:AGEs呈时间依赖方式增加HRMC对RANTES的分泌.

Objective To explore the effects of advanced glycosylation end products (AGEs) on the expression of regulated upon activation, normal T cell expressed and secreted (RANTES) in cultured human renal mesangial cells (HRMC). Method AGEs-BSA was prepared by incubating bovine serum albumin (BSA) with glucose. The cultured HRMC (10 6) were intervened with the same concentrations of AGEs-BSA and BSA. The RANTES level in supernatant was measured by ELISA. Result Compared with the BSA group, the concentration of RANTES in medium began to increase at 8-h and reached the peak at 48-h after intervention with AGEs-BSA ( 640 mg·L -1) (P< 0.01). Conclusion It seems that AGEs-BSA stimulates the secretion of RANTES in cultured HRMC in a time-dependent manner.

参考文献：

[1] Wang S N, laPAGE J, HIRSCHBERG R. Role of glomerular ultrafiltration of growth factors in progressive interstitial fibrosis in diabetic nephropathy. 2000. doi:10.1046/j.1523-1755.2000.00928.x
[2] Singh R, BARDEN A, Mori T. Advanced glycation end products:a review. 2001. doi:10.1007/s001250051591
[3] 孙子林, 刘乃丰, 刘必成. 小分子AGE-肽在小鼠糖尿病肾病发生中的作用. 南京铁道医学院学报1999(3)
[4] 孙子林, 刘乃丰, 弓玉祥. 糖基化终产物一牛血清白蛋白的制备和纯化. 铁道医学1999(6). doi:10.3969/j.issn.1671-7562.1999.06.003
[5] Rovin B H, SCHREINER G F. Cell-mediated immunity in glomerular disease. 1991. doi:10.1146/annurev.me.42.020191.000325
[6] Young B A, JOHNSON R J, ALPERS C E. Cellular events in the evolution of experimental diabetic nephropathy. 1995. doi:10.1038/ki.1995.139
[7] Bacon K B, PREMACK B A, GARDNER P. Activation of dual T cell signaling pathways by the chemokine RANTES. 1995(5231). doi:10.1126/science.7569902
[8] Kuna P, REDDIGARI S R, SCHALL T J. RANTES,a monocyte and T lymphocyte chemoatactic cytokine releases histamine from human basophils, 1992
[9] SCHALL T J, BACON K, TOY K J. Selective attraction of monocyte and T lymphocytes of memory phenotype by cytokine RANTES. 1990. doi:10.1038/347669a0
[10] Natori Y, SEKIGUCHI M, NATORI Y. Expression of mRNA of C-C chemokines in experimental crescentic glomerulonephritis, 1995

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录