实验性小鼠前胃癌肝转移模型的建立-东南大学学报医学版

实验性小鼠前胃癌肝转移模型的建立

单位：1.河南省卫生防疫站,河南郑州,450003； 2.郑州大学医学院,河南郑州,450052； 3.东南大学附属中大医院肿瘤科,江苏南京,210009

分类号：R735.2, R735.7, R73-37, R-332

出版年·卷·期（页码）：2003·22·第五期（313-315）

摘要：

目的:建立实验性小鼠前胃癌肝转移模型.方法:将浓度为1×107个*ml-1的小鼠前胃鳞癌细胞(MFC)悬液0.2 ml接种于615小鼠脾被膜下,分别于术后第1、2、3、4周处死2～3只小鼠,观察腹腔内肿瘤生长情况,其余小鼠待其自然死亡,观察生存天数.结果:肝转移发生率为83.33%,小鼠平均荷瘤生存时间为(37.83±7.32)d.病理结果提示肝转移瘤细胞与脾脏肿瘤细胞形态学相似,符合低分化鳞癌的特征.结论:本研究成功建立了实验性小鼠前胃癌肝转移模型,为筛选抗肿瘤药物和研究肿瘤转移行为提供了有用的模型.

Objective To establish a preventriculus squamous carcinoma liver metastases model for experimental studies in mice.Methods Liver metastases were established in 615 mice by intrasplenic injection of preventriculus squamous carcinoma cells(MFC) in a concentration of 2×10 6 cells in 0 2 ml solution.After surgery,2～3 mice were sacrificed each week,and the others were used to observe the survival time.The liver metastases were investigated pathologically in all animals.Results The incidence of liver metastases was 83 33%,the average survival time of tumor bearing animals was (37 83±7 32) days.The pathological results showed that tumor cells of liver metastases were morphologically similar with spleen tumor cells in a accordance with poorly differentiated preventriculus squamous carcinoma.Conclusion This study successfully established the model of preventriculus squamous carcinoma liver metastases in mice.It’s a useful model to select antineoplastics and to study metastatic behaviors.

参考文献：

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[2] 黄平, 楼荣灿, 张学栋. 脾切除法建立裸小鼠人结肠癌肝转移模型, 1999(3)
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[4] 薛克勋, 高进, 赵雪梅. 人和动物肿瘤移植于免疫缺陷和正常小鼠脾内后肝转移形成及其机制探讨, 1992(4)

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