Among the splicing factors of eukaryon,SR protein family is very important.They are bound to some specific RNA sequences and help to recognize and select correct 5′ and 3′ splice sites,interacting with other splicing factors to finish the work.SR proteins play an important role in determination of the specificity of tissue cells and definition of the stages of individual development.
参考文献:
[1] CHANDLER S D, MAYEDA A, YEAKLEY J M. RNA splicing specificity determined by the coordinated action of RNA recognition motif in SR protein. 1997(8) [2] Misteli T, CACERES J F, CLEMENT J Q. Serine phosphorylation of SR protein is required for their recruitment to sites of transcription in vivo, 1998(2) [3] Yeakley J M, TRONCHERE H, OLESEN J. Phosphorylation regulates in vivo interaction and molecular targeting of serine/arginine-rich pre-mRNA splicing factors. 1999(3) [4] Tacke R, CHEN Y, MANLEY J L. Sequence-specific RNA binding by an SR protein required RS domain phosphorylation:creation of an SRp40-specific splicing enhancers, 1997(4) [5] Zhu J, KRAINER A R. pre-mRNA splicing in the absence of an SR protein RS domain, 2000(24) [6] ELDRIDGE A G, LI Y, SHARP P A. The SRm160/300 splicing coactivator is required for exon-enhancer function. 1999(11). doi:10.1073/pnas.96.11.6125 [7] KANOPKA A, MUHLEMANN O, AKUSJARVI G. Inhibition by SR proteins of splicing of a regulated adenovirus pre-mRNA. 1996(6582). doi:10.1038/381535a0 [8] Fu X D, MANIATIS T. The 35-KDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleuoprotein particles at the 3′ splice site, 1992(5) [9] JUMMA H, WEI G, NIELSEN P J. Blastocyst formation is blocked in mouse embryo lacking the splicing factor SRp20. 1999(9). doi:10.1016/S0960-9822(99)80394-7 [10] Longman D, JOHNSTONE I L, CACERES J F. Functional characterization of SR and SR-related genes in Caenorhabditis elegans. 2000(7) [11] SKICKELER E. Stage-specific changes in SR splicing factors and alternative splicing in mammary tumorigenesis. 1999(24). doi:10.1038/sj.onc.1202671 [12] Zahler A M, NEUGEBAUER K M, LANE W S. Distinct functions of SR proteins in alternative pre-mRNA splicing. 1993(5105). doi:10.1126/science.8385799 [13] LEMAIRE R, PRASAD J, KASHIMA T. Stability of a pKCI-1-related mRNA is controlled by the splicing factor ASF/SF2:a novel function for SR proteins. 2002(5). doi:10.1101/gad.939502 [14] Morrison M, HARRIS K S, ROTH M B. smg mutants affect the expression of alternatively spliced SR protein mRNAs in Caenorhabditis elegans. 1997(18)