火棉胶增强技术在胆囊结石石蜡薄切片中的应用-东南大学学报医学版

火棉胶增强技术在胆囊结石石蜡薄切片中的应用

作者：叶生爱¹ 蒋静娟² 秦永林¹ 芮宗道¹ 陈卫东¹ 范新¹ 王凤臣¹ 汤文浩¹

单位：1.东南大学附属中大医院,普外科,江苏,南京,210009； 2.东南大学附属中大医院,病理科,江苏,南京,210009

分类号：Q-336, R361.2, R575.620.2

出版年·卷·期（页码）：2003·22·第四期（230-231）

摘要：

目的:评估火棉胶增强技术在胆囊结石切片中的应用.方法:收集不同类型的胆囊胆固醇结石20枚,固定后制成石蜡块,采用火棉胶增强技术制作切片.结果:采用此方法制作各类胆囊结石切片100余张(4 μm厚),均获得完整无损的切片.结论:火棉胶增强技术是制作胆囊结石切片一种较好的方法.

Objective To evaluate the collodion-enhancing technique for thin paraffin sections of the gallstones.Method Gallstones (n=20)of different types were embeded in the paraffin after being fixed and dehydrated.Then the paraffin blocks were sectioned by the collodion-enhancing technique.Result This technique resulted in 100 perfect pieces of sections (4?μm).Micro-network structures of mucopolysaccharides were detected after being stained with periodic acid schiff’s (PAS).Conclusion The collodion-enhancing technique is a satisfactory method for sectioning gallstones.

参考文献：

[1] Nakamura. Resin-reinforcing technique for sectioning gallstone, 1966
[2] 戴显伟, 陈淑珍. 胆结石石蜡薄切片的树脂增强技术, 1984(1)
[3] MORIYASU A, ISE H, SUZUKI N. A new technique for thin sections of the gallstones, microscopic detection of mucopolysaccharides in gallstone, 1991(3)
[4] CULLING C F A, 孔庆雷. 组织病理学与组织化学技术手册, 1982

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