RP-HPLC法测定环孢素A脂质体乳胶剂中环孢素A的含量-东南大学学报医学版

RP-HPLC法测定环孢素A脂质体乳胶剂中环孢素A的含量

单位：东南大学附属中大医院药剂科,江苏南京,210009

分类号：O652.63, R927.2

出版年·卷·期（页码）：2003·22·第三期（155-156）

摘要：

目的:用反相高效液相色谱(RP-HPLC)法测定环孢素脂质体乳胶剂中环孢素A的含量.方法:以Spherisorb C8(5μm,200mm×4.6mm ID)为色谱柱,乙腈-pH2磷酸盐缓冲液(68∶32)为流动相,检测波长UV210nm,流速1.0ml*min-1,柱温60℃.结果:环孢素A在10～400mg*L-1浓度范围内线性关系良好,回收率为99.52%,相对标准差为1.62%(n=5).结论:RP-HPLC法准确,灵敏度高,重现性好,可用于测定环孢素A的含量.

Objective A rapid and sensitive reversed phase high performance liquid chromatographic method for the determination of cycloporine A in cycloporine A liposomes cream has been developed.Methods The mixture of pH2 potassium phosphate buffer?acetonitrile(68∶32) was used as a mobile phase,while a Spherisorb C 8 column (5?μm,200?mm× 4.6 ?mm?ID) was used as a stationary phase, and CsA was detected at 210?nm with flowing rate being rate 1.0ml·min -1 and column temperature at 60?℃.Results CsA had a good linear relation in the range of 10～400?mg·L -1 ,the mean recovery of was 99.52 %,the RSD was 1.62%( n =5).Conclusion The result showed that this method was reliable and accurate and it can be applied to the content determination of cycloporine A in cycloporine A?liposomes cream.

参考文献：

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