聚合酶链反应和DNA探针杂交法检测沙眼衣原体的比较-东南大学学报医学版

聚合酶链反应和DNA探针杂交法检测沙眼衣原体的比较

单位：1.东南大学附属中大医院检验科,江苏南京,210009； 2.南京军区南京总医院全军医学检验中心,江苏南京,210002

分类号：R374.1, R711.32

出版年·卷·期（页码）：2003·22·第一期（35-37）

摘要：

目的:探讨聚合酶链反应(PCR)和DNA探针杂交法检测泌尿生殖道沙眼衣原体(Ct)的临床诊断价值.方法:应用这两种方法对未使用过抗衣原体药物的80例宫颈炎患者同时进行Ct检测.结果:PCR法检出Ct基因的阳性率(23.8%)高于DNA探针杂交法(15.0%),两者比较有显著性差异.结论:PCR法检测Ct较DNA探针杂交法敏感,如将两法结合应用结果将更可信.

Objective To explore the clinical diagonstic value of polymerase chain reaction (PCR) and DNA probe hybridization for detecting Chlamydia trachomatis (Ct) infection of urogenital tract.Method 80 specimens from cervicitis subject were examined by PCR and DNA probe hybridization simultaneously.Result The positive rate of PCR (23.8%) was higher than that of DNA probe hybridization (15.0%) significantly.Conclusion PCR is more sensitive than DNA probe hybridization in detecting Ct infection,and the combination of two would be more preferable.

参考文献：

[1] WITKIN S S, JEREMIAS J, GRIF J A. Detection of chlamydia trachomatis by the polymerase chain reaction in male members of infertile couples, 1993(5)
[2] Cates W Jr, WASSERHEIT J N. Genital chlamydial infections:epidemiology and reproductive sequelae, 1991(6)
[3] BRAVERMAN P K, STRASBURGER V C. Sexually transmitted diseases, 1994(1)
[4] 叶顺章, 宦秉瑜, 钟镜增. 我国部分地区性病高危人群生殖道衣原体感染和HIV抗体的调查, 1993(4)
[5] CLASS H C J, WAGENVOORT J H T, NIESTERS H G M. Diagnostic value of the polymerase chain reaction for chlamydia detection as determined in a follow-up study, 1991(1)
[6] BLACK C M. Current methods of laboratory diagnosis of chlamydia trachomatis infections. 1997(1)
[7] KLUYTMANS J A J W, NIESTERS H G M, MOUTON J W. Performance of a nonisotopic DNA probe of detection of chlamydia trachomatis in urogential specimens, 1991(12)
[8] Paukku M, PUOLAKKAINEN M, APTER D. First-void urine testing for chlamydia trachomatis by polymerase chain reaction in asymptomatic women. 1997(6). doi:10.1097/00007435-199707000-00006
[9] TABRIZI S N, CHEN S, BORG A J. Patient-administered tampon-collected genital cells in the assessment of chlamydia trachoma tis infection using polymerase chain reaction. 1996(6). doi:10.1097/00007435-199611000-00010
[10] GOESSENS W H, KLUYTMANS J A, Den TOOM N. Influence of volume of sample processed on detection of chlamydia trachomatis in urogenital samples by PCR. 1995(1)

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