新型融合毒素hIL-15-TNFα表达质粒的构建-东南大学学报医学版

新型融合毒素hIL-15-TNFα表达质粒的构建

单位：东南大学基础医学院生物化学教研室,江苏南京,210009

分类号：Q784, R392.2, R730.3

出版年·卷·期（页码）：2003·22·第一期（22-25）

摘要：

目的:构建hIL-15-TNFα融合毒素的表达质粒,为该融合毒素的功能研究及临床应用前景的分析评价奠定基础.方法:将hIL-15成熟肽基因与人工改造的TNFα基因按正确的阅读框架融合,定向克隆于表达载体pET16b.结果:经酶切分析及DNA序列分析,所构建的质粒均插入hIL-15-TNFα融合基因.结论:利用pET16b表达载体可成功构建融合毒素hIL-15-TNFα的表达质粒.

Objective To characterize the biological function and clinical perspective of hIL?15?TNF α fusion toxin,a plasmid expressing this fusion molecule was constructed.Method Artifically modified TNF α cDNA was fused precisly to the reading frame of hIL?15 and further cloned into the E.coli expression vector pET16b.Results The recombinant plasmid containing hIL?15?TNF α fusion gene was obtained as demonstrated by restriction analysis and DNA sequencing.Conclusion The recombinant plasmid expressing hIL?15? TNF α fusion toxin is successfully constructed.

参考文献：

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