应用荧光定量PCR检测血清中HCV RNA-东南大学学报医学版

应用荧光定量PCR检测血清中HCV RNA

单位：1.东南大学基础医学院病原生物与免疫学系,江苏南京,210009； 2.东南大学附属中大医院检验中心,江苏南京,210009； 3.东南大学遗传学研究中心,江苏南京,210009

分类号：Q503, R512.63, R466.9

出版年·卷·期（页码）：2003·22·第一期（16-18）

摘要：

目的:探讨荧光定量PCR(FQ-PCR)法检测血清中丙型肝炎病毒(HCV)含量的敏感性、特异性.方法:采用FQ-PCR和逆转录巢式PCR同步检测76份HCV抗体阳性血清标本,并对两种方法的检测结果进行对照比较.结果:FQ-PCR定量HCV RNA拷贝数在102～106拷贝*μl-1,其中低于103拷贝*μl-1占20.93%,103～105拷贝*μl-1占60.47%,高于105拷贝*μl-1占18.60%.76份HCV抗体阳性血清中,巢式PCR检测阳性为45份,FQ-PCR检测阳性为43份,二者相对符合率为97.37%.结论:FQ-PCR检测HCV RNA与常规定性巢式PCR特异性、敏感性基本一致.

Objective To explore the sensitivity and the specificity of fluorescence quantitative PCR(FQ-PCR) by which HCV RNA level of serum is determined.Methods HCV RNA in serum was determined by RT nested PCR and FQ?PCR simultaneously.Results The copies of HCV RNA ranged from 10 2 copies·μl -1 to 10 6 copies·μl -1 ,among which,20.93% was below 10 3 copies·μl -1 ,60.47% was between 10 3 copies·μl -1 and 10 5 copies·μl -1 ,the rest was more than 10 5 copies·μl -1 .In 76 antibody positive samples,45(60.8%) was nested PCR positive,43(58.1%) was FQ?PCR positive,showing no statistical difference.Conclusions The level and distribution of HCV RNA in Nanjing is the same as in other places reported in literature.FQ?PCR method has the same sensitivity and specificity as RT?nested?PCR.

参考文献：

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