目的:探讨CD40、CD40L在人单核细胞及在动脉粥样硬化斑块中共同表达的意义.方法:CD40及CD40L在单核细胞表面的表达分别采用荧光技术、RT-PCR、流式细胞仪和Western blotting检测.人的动脉粥样硬化斑块中CD40及CD40L的表达采用免疫组织化学方法.结果:人单核细胞能连续表达CD40及CD40L,并且细胞因子IL-1、IL-6、TNF和INF-γ能明显上调单核细胞表达CD40、CD40L的水平.人动脉粥样硬化斑块中能共同表达CD40及CD40L,而动脉壁的其它部分不表达.结论:人单核细胞表面及粥样硬化斑块中能共同高表达CD40和CD40L,提示CD40及CD40L相互作用在动脉粥样硬化形成及其发展中起重要作用.

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