目的:研究β肾上腺素能受体(β受体)激动是否增加人血小板一氧化氮合酶(NOS)活性.方法:利用Sepharose法分离血小板,根据NOS能转换L-精氨酸为L-瓜氨酸的原理,观察基础状态下给予NOS阻滞剂左旋亚硝基精氨酸甲基酯(L-NMMA)、激动剂组胺和β受体激动剂异丙肾上腺素后NOS活性变化,以及选择性β受体亚型阻断后对异丙肾上腺素的影响.结果:基础状态下人血小板有一氧化氮释放,L-NMMA能明显抑制这种释放;组胺和异丙肾上腺素均能明显增加血小板NOS活性,β2受体阻断剂能完全阻断异丙肾上腺素增加NOS活性的作用.结论:异丙肾上腺素能增加血小板NOS活性,这种作用通过β2受体介导.

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