RP-HPLC法测定格替沙星片中格替沙星的含量-东南大学学报医学版

RP-HPLC法测定格替沙星片中格替沙星的含量

单位：1.东南大学附属中大医院,药剂科,江苏,南京,210009； 2.南京泽辰科技有限公司,江苏,南京,210009

分类号：R927.2

出版年·卷·期（页码）：2002·21·第三期（232-234）

摘要：

目的:用反相高效液相色谱(RP-HPLC)法测定格替沙星片中格替沙星的含量.方法:以Shimpack CLC-ODS C18 (5 μm,150 mm×4.6 mm)为色谱柱,0.02 mol*L-1磷酸二氢钾缓冲液-乙腈(85∶15)为流动相;紫外检测波长为293 nm,流速为1.0 ml*min-1.结果:格替沙星在4～32 mg*L-1浓度范围内线性关系良好,平均加样回收率为99.8%,相对标准差为1.05%(n=5).结论:RP-HPLC法准确、灵敏度高、重现性好,可用于测定格替沙星片剂的含量.

Objective To determine gatifloxacin in tablets with a rapid and sensitive reversed phase high performance liquid chromatographic method.Methods The mixture of 0.02?mol·L -1 monobasic potassium phosphate buffer acetonitrile (85∶15) was used as a mobile phase,while a Shimpack CLC?ODS C 18 column (5?μm,150?mm×4.6?mm) was used a stationary phase,and gatifloxacin was detected at 293?nm with flowing rate 1.0?ml·min -1 .Results Gatifloxacin had a good linear relation in the range of 4～32?mg·L -1 ,the mean recovery of was 99.8%,the RSD was 1.05 % ( n =5).Conclusion The results show that this method is reliable and accurate and it can be applied to the content determination of gatifloxacin tablets.

参考文献：

[1] HOSAKA M A, YASUE T O, FUKUA. In vitro antibacterial activities of AM-1155,a new 6-fluoro-8-methoxyquinolone, 1992(10)
[2] WAKABAYASSHI E, MITSUHASHI S. In vitro anthibacterial activity of AM-1155,a novel 6-fluoro-8-methoxy quinolone, 1994(3)
[3] KUNINISHI Y, NAGATA M, OMOTERA A. Phototoxicity studies of gatifloxacin in guinea pigs and mice, 1998(10)
[4] KUNINISHI Y, NOMO Y, TSURU K. Photosensitization study of gatifloxacin in guiea pigs, 1998(10)
[5] Kusajima H, ISHIDA R, UCHIDA H. Phototoxicity of gatifloxacin,a new quinolone,and its related drugs in guiea pigs, 1998(10)
[6] Nakashima M, UEMATSUGE K, KUSAJIMA H. Single and multiple-dose pharmacokinetics of AM-1155,a new 6-fluoro-8-methoxy quinolone in humans, 1995(12)

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