CyclinD1、P16和P21WAF1/CIP1在胃癌中的表达意义-东南大学学报医学版

CyclinD1、P16和P21WAF1/CIP1在胃癌中的表达意义

单位：1.浙江大学医学院附属第二医院,普外科,浙江,杭州,310009； 2.山东省立医院,普外科,山东,济南,250013

分类号：Q786, R735.2

出版年·卷·期（页码）：2002·21·第三期（213-216）

摘要：

目的:探讨癌基因CyclinD1,抑癌基因p16、p21与胃癌发生的生物学行为的关系.方法:采用免疫组化LSAB法测定43例胃癌CyclinD1、P16和P21WAF1/CIP1蛋白的表达.结果:CyclinD1在胃癌组织中的阳性率为55.81%,CyclinD1与胃癌分化程度呈正相关(P<0.05);P16和P21在胃癌组织中阳性率分别为48.84%和46.51%,P16和P21与胃癌分化程度均呈负相关(P<0.05);CyclinD1与P16、P21分别呈负相关;P16与P21无相关性;CyclinD1、P16和P21与胃癌的预后相关.结论:CyclinD1的高表达,P16和P21的低表达在胃癌的发生发展中起重要作用,并可能成为研究胃癌发生、发展的有用指标.

Objective To investigate the expression of CyclinD 1,P16 and P21 protein in human gastric carcinoma,to explore the correlation of these proteins expression with biologic behavior of tumors.Method CyclinD 1,P16 and P21 protein expression were studied in formalin fixed,paraffin embedded sections of gastric carcinoma by means of a specific and sensitive immunohistochemical LSAB assay.Results The positive rate of CyclinD 1 in gastric carcinoma tissuse was 55.81%.The expression of CyclinD 1 was positively correlated with grade of tumor differentiation( P <0.05).The positive rate of P16 in gastric carcinoma tissuse was 48.84%.The positive rate of P21 in gastric carcinoma tissuse was 46.51%.The presence of P16 and P21 was negatively correlated with grade of tumor differentiation( P <0.05).There are a high negatively correlation between the expression of CyclinD 1 and P16,between CyclinD 1 and P21.P16 and P21 were non relation.The expression of CyclinD 1,P16 and P21 protein were correlated with the prognosis of patients with gastric carcinoma.Conclusion The overexpression of CyclinD 1,and depressed expression of P16 and P21 are contributing to the occurrence and development of gastric carcinoma.and they can be useful parameteres in studying the occurrence and development of gastric carcinoma.

参考文献：

[1] Thomas D G, GELEHRTER D G, FRANCIS S C. Principles of medical genetics, 1990
[2] Morsaki T, UCHIYAMA A, Yuzuki D. Interleukin 4 regulates G1 cell cycle progression in gastric carcinoma cells, 1994(4)
[3] Ogawa M, MAEDA K, noda N. Immunohistochemical study of P21 expression in gastric carcinoma, 1997(Suppl 2)
[4] Akama Y, YASUI W I, KUNIYAS H I. Genetic status and expression of the cyclin-dependent kinase inhibitors in human gastric carcinoma cell lines, 1996(8)
[5] 吕有勇, 高崇峰, 崔建涛. 胃癌中MTS1/p16基因缺失及表达异常的研究, 1996(3)
[6] Yokota J. Multiple steps in carcinogenesis involving alternations of multiple tumor suppress gene, 1993(10)

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