大鼠胰十二指肠移植动物模型的显微外科技术改进-东南大学学报医学版

大鼠胰十二指肠移植动物模型的显微外科技术改进

单位：1.南京铁路中心医院,江苏,南京,210003； 2.南京大学医学院,江苏,南京,210008

分类号：R-332, R656.6, R657.5

出版年·卷·期（页码）：2002·21·第二期（153-154）

摘要：

目的:改进大鼠胰十二指肠移植动物模型,控制失血性休克的发生,提高术后成功率.方法:供体门静脉同受体左肾静脉采用"卡夫"管相吻合,避免术中受体肝素化而导致血管吻合口渗血.给受体输注供体新鲜血2.5～3 ml.结果:药物诱导糖尿病大鼠行胰十二指肠移植65例中60例成活超过7 d,成功率92.3%;非禁食情况下,血糖浓度均正常.结论:该模型的技术改进减少了许多并发症,大大提高了手术成功率.

Objective To refine the technique of Lee’s pancreaticoduodenal transplantation in the rat,to overcome hypovolemic shock,to enhance the success rate.Methods The venous anastomosis was reconstructed by a "cuff" anastomosis between the donor portal vein and the recipient left renal vein.Meanwhile,the recipient was injected 2.5～3?ml fresh blood which was taken from the donor.Result Of 65 transplanted rats that were induced diabetes,60 rats survived over 7 days,the success rate was 92.3%.All success rats were euglycemia.Conclusion The refined techniques could eliminate most surgical complications,allowing the pancreaticoduodenal transplantations in the rat with a high degree of success.

参考文献：

[1] Lee S, TUNG K S, KOOPMANS H. Pancreaticodudenal transplantation in the rat. 1972. doi:10.1097/00007890-197204000-00010
[2] Zhong R, GRANT D, SUTHERLAND F. Refined technique for intestinal transplantation in the rat. 1991. doi:10.1002/micr.1920120408
[3] Radziak J, BARRON P, NAJM H. The effect of systemic venous drainage of pancreas on insulin sensitivity in dogs. 1993. doi:10.1172/JCI116758
[4] Kononov A, BROWNE E Z, ALEXANDER F. Continuous rat intravenous infusion. 1994. doi:10.1002/micr.1920150615

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