烹调油烟职业暴露人群遗传损伤的分子流行病学研究-东南大学学报医学版

烹调油烟职业暴露人群遗传损伤的分子流行病学研究

单位：东南大学公共卫生学院,江苏南京,210009

分类号：R181.34

出版年·卷·期（页码）：2002·21·第一期（123-126）

摘要：

目的:对职业暴露于烹调油烟的人群进行生物标志物检测和分析,以探讨烹调油烟对机体的遗传损伤作用.方法:以外周血淋巴细胞姊妹染色体交换(SCEs)和微核(MN)为标志物,比较暴露组与对照组标志物的差异及影响因素.暴露组为46名男性饭店厨师,对照组为28名男性饭店管理人员.结果:暴露组的标志物水平明显高于对照组.暴露组SCEs的平均值为7.37,对照组为4.17.协方差分析调整吸烟、饮酒等混杂因子后,两组SCEs仍然存在显著性差异(P=0.0001).多因素逐步回归分析表明,暴露组人群的SCEs水平随暴露年限的延长而升高.暴露组微核检出阳性率和微核细胞率分别为52.17%和0.237%,都明显高于对照组的17.86%和0.075%.结论:烹调油烟对职业暴露人群具有一定的遗传损伤作用.

Objective To estimate the biological changes and biomarkers in those occupationally exposed to cooking fumes.Methods Sister chromatid exchanges(SCEs) and micronucleus(MN) in peripheral blood lymphocytes were used as biomarkers.The exposure group( n =46) consisted of 46 male cooks who were empolyed in restaurants and inhaled cooking fumes every day.Controls( n =28),matched for sex,age,smoking habits,alcohol consumption,consisted of 26 male managers.Students t test was used to compare the frequency of SCEs and MN in exposed workers and controls.The relationship between the biomarkers and exposure to cooking fumes was also assessed by multiple linear regression.Results The exposure related changs of biomarker in exposed group were significantly higher than those in control group.Mean value of SCEs in exposed group was found to be 7.37 and 4.17 in control group.The positive rate for micronucleus test in the exposed group was significantly higher than that in the controls(52.17% and 17.86,respectively, p =0.003),The micronucles per cell in the exposed group was 0.237%,and was significantly higher than that in the controls(0.075%, P <0.01).The mean frequency of SCEs in the exposed group was 7.37 and was significantly higher than that in the controls(4.17).After adjusting the confoundings such as smoking,alcohol intake,the analysis of covariance revealed that the frequency of SCEs was significantly higher than that in controls ( P =0.000?1).Multiple linear regression analysis(using a continuous exposure variable)suggested that the Ln transformation frequency of SCEs increased with exposure years in the exposed group.Conclusion Occupational exposure to cooking fumes might result in a certain genetic damage.

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