不同基因型戊型肝炎病毒重组蛋白的抗原性分析-东南大学学报医学版

不同基因型戊型肝炎病毒重组蛋白的抗原性分析

作者：孟继鸿¹ 戴星² 窦晓光³ Y E KHUDYAKOV⁴ H A FIELDS⁴

单位：1.东南大学基础医学院病原生物学和免疫学系,江苏南京,210009； 2.东南大学附属中大医院皮肤科,江苏南京,210009； 3.中国医科大学附属第二医院感染病科,辽宁沈阳,110004； 4.美国疾病控制和预防中心肝炎部,Atlanta,Georgia,30333

关键词：戊型肝炎病毒基因型重组蛋白抗原性

分类号：R373.21, R446.6

出版年·卷·期（页码）：2002·21·第一期（31-35）

摘要：

目的:探讨不同基因型和亚型戊型肝炎病毒(HEV)重组蛋白氨基酸序列的变化对HEV抗原性的影响,筛选出最佳的HEV抗原用于戊型肝炎的免疫诊断.方法:用已知序列的HEV基因工程表达蛋白作为包被抗原,对已知血清标本进行酶联免疫吸附试验测定.结果:6种不同抗原对30份正常献血者血清无抗原性,对17份急性戊型肝炎病人血清和5份实验感染动物血清均呈阳性反应,但血清抗体滴度的高低与所用抗原的基因型有关,尤其是受到该抗原第76位氨基酸变化的影响.结论:多基因型HEV抗原混合物是HEV免疫诊断的最佳抗原.

Objective To explore the effects of amino acid variation of recombinant proteins of the different genotypes and subtypes of hepatitis E virus (HEV) on the antigenicity of HEV antigen and to screen the best HEV antigen for hepatitis E immunodiagnosis.Method Applying the HEV recombinant proteins with known sequences as the coated antigens, the serum samples with known background were tested by ELISA.Results No antigenicity for all the 6 different antigens was found to 30 serum samples obtained from normal blood donors.Positive reactions to each of these antigens were observed for all of the 17 serum specimens obtained from patients with acute hepatitis E and 5 serum samples from experimently infected animals.However,the titers of the anti HEV antibody were associated with the genotypes of the antigens applied in the assay, especially were determined by the variation of the amino acid located at the position 76 within the amino acid sequence of the HEV recombinant proteins.Conclusion The mixture of multi genotype HEV antigens is the best one for HEV immunodiagnostics.

参考文献：

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