脑血管痉挛大鼠血和脑组织ET 1、NO含量动态变化的研究-东南大学学报医学版

脑血管痉挛大鼠血和脑组织ET 1、NO含量动态变化的研究

单位：1.东南大学医学院,生理学教研室,； 2.泰山医学院附属医院,微循环研究所,

分类号：R743.35, R332

出版年·卷·期（页码）：2001·20·第四期（222-224）

摘要：

目的:探讨内皮素(ET) 1和一氧化氮(NO)在蛛网膜下腔出血(SAH)后脑血管痉挛(CVS )及脑缺血性损害中的作用.方法:采用非开颅法大鼠SAH模型,检测24 h内血及脑组织中NO和ET 1含量,并测定脑微区血流(rCBF)和基底动脉管径. 结果:大鼠SAH后30 min基底动脉管径缩小,rCBF持续下降;血NO减少,ET 1增多;脑组织NO和ET 1均增加.结论:血NO减少,ET增多系SAH后CVS发生的原因之一;脑组织NO和ET增多则加重脑损伤.

Objective To investigate the possible roles of endothelin?1 (ET?1)and nitric oxide (NO) in the development of cerebral vasospasm(CVS) and related brain ischemic damage following subarachnoid hemorrhage (SAH).Methods 90 Wistar rats were divided into SAH group and sham operated group. Regional cerebral blood flow (rCBF),NO and ET?1 in both blood and brain tissue within 24?h after operation were detected.Diameter of basilar artery(BA) was measured.Results rCBF decreased rapidly and persistently within 24?h after induction of SAH. Spasm of BA occurred 30?min after SAH.NO in blood was decreased while that in brain tissue increased. Both blood and brain tissue ET?1 levels were increased.Conclusion Decrease of NO and increase of ET?1 in blood are involved in the development of CVS following SAH while increase of NO and ET?1 in brain tissue contributes to brain damage due to ischemia.

参考文献：

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