局灶性脑缺血再灌注损伤对大鼠延髓内脏带NOS阳性神经元与FOS蛋白表达的影响-东南大学学报医学版

局灶性脑缺血再灌注损伤对大鼠延髓内脏带NOS阳性神经元与FOS蛋白表达的影响

单位：东南大学基础医学院,解剖学教研室,

分类号：R322.8, R743

出版年·卷·期（页码）：2001·20·第四期（211-215）

摘要：

目的:观察大鼠局灶性脑缺血4 h再灌注不同时间对延髓内脏带(MVZ)内一氧化氮合酶(NOS)阳性神经元分布及FOS蛋白表达的影响,探讨NOS阳性神经元与FOS蛋白表达的变化及相互关系 ,阐明在缺血状态下MVZ的功能作用机制.方法:以大脑中动脉线栓法建立局灶性脑缺血再灌注模型,采用NADPH d组织化学(组化)反应、FOS蛋白免疫组化反应及双重染色技术显示MVZ在不同时相的3种阳性神经元变化,并以假手术组和正常对照组作对照.结果:脑缺血组MVZ内NOS阳性神经元染色反应增强和FOS蛋白表达随脑缺血后再灌注时程而变化 ,并见有15%～20%阳性双染细胞.结论:在局灶性脑缺血再灌注损伤过程中,MVZ内NOS阳性神经元与FOS蛋白表达的变化具有相关性,提示MVZ参与机体的应激反应和保护性机制.

Objective To observe the effects of distribution of nitric oxide synthase(NOS) positive neurons and FOS protein expression in the rat medullary visceral zone (MVZ) following focal cerebral ischemia reperfusion injury.To study the correlative change between NOS positive neurons and FOS protein expression and illustrate the function role of MVZ in the ischemia injury.Methods Nylon thread was inserted into the middle cerebral artery in rats to produce an animal model of focal cerebral ischemia reperfusion.Using NADPH?d histochemical and anti?FOS protein immunohistochemical and NOS/FOS double contained methods displayed the expressing changes of the three positive neurons at different time periods in MVZ were investigated.Brain slices of sham operated group and normal group were taken for control.Results Both the containing reactions of NOS positive neurons were enhanced and FOS protein expression changed with the changes of time course after ischemia reperfusion.Approximately 15～20 percent of the double contained cells showed in medullary visceral zone.Conclusion The result indicated that the change of distribution of NOS positive neurons was positively related with FOS protein expression and was associated with regulation or control of the stress and defence responses in the rat MVZ after cerebral ischemia.

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