Objective To introduce a new method to culture corneal endothelial cells.Methods Through stero microscope,Descemet’s membrane and endothelial cell layer were torn apart and cut out into small pieces,then the pieces were put into culture containers and coverglasses were piled up on tissue pieces for culture.Results The corneal endothelial cells were transformed quickly from tissue pieces.Conclusion Enzyme is not required in this method,moreover,the method has many advantages,such as simple,reliable,pollution proof and high yield.In a word,it is a good method worth recommending. |
