23-羟基桦木酸体外抗恶性肿瘤作用的研究-东南大学学报医学版

23-羟基桦木酸体外抗恶性肿瘤作用的研究

单位：1.东南大学医学院现代医学实验中心； 2.中国药科大学天然化学教研室

分类号：R965

出版年·卷·期（页码）：2001·20·第三期（141-144）

摘要：

目的:研究23-羟基桦木酸在体外对B16、SGC-7901、A0、HL-60、K562、HeLa及S180 7种恶性肿瘤细胞生长的抑制作用.方法:应用四甲基偶氮唑盐比色分析、电镜观察、流式细胞术等方法观测23-羟基桦木酸对7种肿瘤细胞的抑制作用.结果:10～160 mg*L-1的23-羟基桦木酸在体外对7种肿瘤细胞有显著的抑制作用,呈一定的剂量依赖关系,并可引起大量肿瘤细胞凋亡.23-羟基桦木酸-Ⅰ使B16肿瘤细胞被阻滞在G0-G1期,而23-羟基桦木酸-Ⅱ则使细胞阻滞在G2-M期.结论:23-羟基桦木酸对体外7种恶性肿瘤细胞均有显著的抑制作用,并使细胞阻滞在某一生长期,最终导致肿瘤细胞凋亡.

Objective To study the growth inhibition in seven cancer cell lines induced by 23?hydroxyl betulinic acid in vitro .Methods 3?(4,5?dimethylthiazole?2?yl)?2,5?diphenyl?tetrazolium bromide(MTT) assay,electronic microscopy and flow cytometry were used to examine the effect of 23?hydroxyl betulinic acid on seven cancer cell lines.Results 23?hydroxyl betulinic acid at the dose of 10～160 mg·L -1 markedly inhibited the growth of seven cancer cell lines in dose dependent man ner,and finally cause a great number of cancer cells apoptosis.23?hydroxyl betulinic acid?Ⅰ result in accumulation of B16 cells by retarding cell cycle at G 0?G 1 phase and 23?hydroxyl betulinic acid?Ⅱ arresting S180 at G 2?M phase.Conclusions 23?hydroxyl betulinic acid significantly inhibited the proliferation of seven cancer cell lines by retarding cells at certain phase of cell cycle and finally caused cells apoptosis.

参考文献：

[1] HESS S C, BRUM R L, HONDA N K. Antibacterial activity and phytochemical analysic of Vochysia divergens(Vochysiaceae). 1995(47). doi:10.1016/0378-8741(95)01260-K
[2] Fujioka T, KASHIWADA Y, KILKUSKIC R E. Anti-AIDS agents 11 betulinic acid and platanic acid as anti HIV principles from syzigium claviflorum,and the anti-HIV activity of structurally related triterpenoids. 1994. doi:10.1021/np50104a008
[3] PISHA E, CHAI H, LEE I S. Discovery of betulinic acid as a selective inhibiter of human melanoma that function by induction of apoptisis. 1995. doi:10.1038/nm1095-1046
[4] 叶银英, 何道伟, 叶文才. 23-羟基桦木酸体外和体内抗黑色素瘤作用的研究. 中国肿瘤临床与康复2000(1). doi:10.3969/j.issn.1005-8664.2000.01.002
[5] Mosman T. Rapid colorimctric assay for cellular grouth and survival:application to proliferation and cytoxicity assays. 1983. doi:10.1016/0022-1759(83)90303-4
[6] Fulda S, SCAFFIDI C, SUSIN S A. Activation of mitochondria and release of mitachondrial apoptogenic factors by betulinic acid. 1998(51). doi:10.1074/jbc.273.51.33942
[7] 林晨, 邓友平, 郑杰. 三氧化二砷诱导人肿瘤细胞凋亡和G2+M期阻滞但引起人永生化宫颈上皮细胞G1期阻滞. 中国医学科学院学报2000(2)

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