Objective To study the effect of drug sera of anti fibrosis compond priscription (ACP) on the functions of primarily cultured normal rat hepatocyte such as proliferation and viability.Methods Primarily cultured normal rat hepatocytes were cultured with regular method, and incubated with “five twenty times” drug sera of ACP for 48?h.The cell proliferation was measured by [ 3H] TdR incorporation, cell viability by [ 3H] Pro incorporation.Results The three drug sera groups could promote the proliferation and viability of normal rat hepatocytes,10%“ten fold” drug sera of ACP showed the best effect.Conclusion The drug sera of ACP could enhance the proliferation and viability of normal rat hepatocytes,the best drug concentration is “ten fold” drug sera of ACP in vitro . |