Objective  The purpose of the present study was to analyze the effect of Gusuibu on the ototoxicity by streptomycin.Methods  A deafness model was established in 30 guinea pigs by streptomycin (300?mg·kg    -1  , i.m.),in which 17 were used as experimental group(32 ears) and 13 as controls(18 ears).The experimental animals were administrated with 100% Gusuibu solution(5?ml·kg    -1  )orally.Cochlear pathology was assessed by surface preparation technique.Detail counts of hair cells were recorded under light microscope and analyzed statistically.Result  Gusuibu decreased the loss of outer hair cells in the first and second coils.Conclusion  Gusuibu may attenuate the ototoxicity by streptomycin.