Objective  This experiment was designed to observe the immunologic functions of CD  3AK cells into which interleukin  2(IL  2) gene had been transducted.Methods  The post  transfer CD  3AK cells’ special Neo  R gene and cell immunologic functions including IL  2 expression,proliferation,cytotoxicity and cell phonetype were detected.Results  The specific 347?bp Neo  R gene was amplified in post  transfer cells.The post  transfer cells expressed higher IL  2, proliferation and cytotoxi~city ability. It was also found that the ratio of CD  4  +T cell to CD  8  +T cell increased in post  transfer group. Conclusion  Transducting IL  2 gene into CD  3AK cells could enhance their immunologic functions.