IGF-1R mRNA在低氧性肺动脉高压大鼠肺组织中的表达-东南大学学报医学版

IGF-1R mRNA在低氧性肺动脉高压大鼠肺组织中的表达

单位：1.南京铁道医学院附属医院呼吸内科,江苏南京,210009； 2.华西医科大学附属第一医院呼吸内科,四川成都,610041

分类号：R-33, R543.2, R563

出版年·卷·期（页码）：2000·19·第三期（168-170）

摘要：

目的:探讨胰岛素样生长因子-1受体(IGF-1R)mRNA在低氧大鼠肺小动脉壁的变化.方法:采用原位杂交方法检测低氧性肺动脉高压大鼠和正常大鼠肺小动脉壁IGF-1R mRNA的表达,用图像分析技术检测肺小动脉的形态改变和半定量技术检测IGF-1R mRNA的表达强度.结果:低氧性肺动脉高压大鼠肺小动脉管壁增厚、管腔狭窄,反映管壁增厚的两个指标MT%和MA%均显著高于正常对照组(P<0.05).正常对照组大鼠肺小动脉壁IGF-1R mRNA有微弱表达,低氧组肺小动脉壁IGF-1R mRNA表达呈强阳性,低氧组表达强度显著大于正常对照组(P<0.001).结论:低氧情况下,肺小动脉壁IGF-1R mRNA表达水平与低氧性肺血管重建密切相关.

Objective The experiment was designed to elucidate the change in insulin like growth factor 1 receptor(IGF 1R)mRNA in the walls of pulmonary arterioles of hypoxic rat.Methods The expression of IGF 1R mRNA was detected by hybridization in situ technique on the walls of pulmonary arterioles in the rats with hypoxic pulmonary hypertension and normal. Morphological change in pulmonary arterioles was determined by image pattern analysis technique, the level of expression of IGF 1R mRNA was detected by semi quantitative technique.Results Compared with the normal control group, the walls of pulmonary arterioles were found to be thickened with both MT% and MA% higher significantly in the rat with hypoxic pulmonary hypertension ( P <0.05,respectively).The expression of IGF 1R mRNA of the pulmonary arterioles in the hypoxic rats was stronger than that in the normal( P <0.001).Conclusion In hypoxic condition, the level of the expression of IGF 1R mRNA in the pulmonary arterioles is closely related to hypoxic pulmonary vascular remodelling.

参考文献：

[1] Jensen D E, Rich C B, Terpstra A J. Transcriptional regulation of the elastin gene by insulin-like growth factor-I involves disruption of Sp1 binding in aortic smooth muscle cells, 1995
[2] Hislop A A, Springall D R, Buttery L D K. Abundance of endothelial nitric oxide synthase in newborn intrapulmonary arteries. 1995(1). doi:10.1136/adc.73.1.17
[3] Raphael R, Renato B. Biology of disease,insulin-like growth factor-Ⅰ receptor,its role in cell proliferation,apoptosis,and tumorigenicity, 1995(3)
[4] DeAngelis T, Gerber A, Baserga R. The insulin-like growth factor Ⅰ receptor is a requirement for the mitogenesis and transforming activities of the platelet-derived growth factor receptor, 1995
[5] Du J, Sperling L S, Marrero M B. G-protein and tyrosine kinase receptor cross-talk in rat aortic smooth muscle cells:thrombin-and angiotensin II-induced tyrosine phosphorylation of insulin receptor substrate-1 and insulin-like growth factor 1 receptor. 1996
[6] Delafontaine P. Insulin-like growth factor I and its binding proteins in the cardiovascular system. 1995
[7] Du J, Delafontaine P. Inhibition of vascular smooth muscle cell growth through antisense transcription of a rat insulin-like growth factor I receptor cDNA, 1995(6)
[8] Moromisato D Y, Moromisato M Y, Stefania Zanconato. Effects of hypoxia on lung,heart,and liver insulin-like growth factor-I gene and receptor expression in the new rat, 1996(6)
[9] Dzau V J, Horiuchi M. Vascular remodeling-the emerging paradigm of programmed cell death(apoptosis). 1998(1). doi:10.1378/chest.114.1_Supplement.91S-a

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