rhC5a对外周血单个核细胞PKC活性的影响-东南大学学报医学版

rhC5a对外周血单个核细胞PKC活性的影响

单位：南京铁道医学院附属医院,临床免疫室,江苏南京,210009

关键词：外周血单个核细胞补体C5a 补体C5a受体蛋白激酶C 佛波脂细胞信号转导

分类号：R392.7

出版年·卷·期（页码）：2000·19·第三期（155-157）

摘要：

目的:观察外周血单个核细胞(PBMC)在rhC5a诱导后蛋白激酶C(PKC)活性变化及其特点.方法:将PBMC与rhC5a置体积分数为0.05的CO2中37℃孵育,按一定时间间隔收集细胞,然后测定PKC活性.结果:PBMC经rhC5a诱导后PKC活性呈双峰型增高.结论:C5a细胞内信号转导过程与PKC活化有关.

Objective The purpose of the study was to observe the shift of activity of protein kinase C(PKC) in peripherial blood mononuclear cell (PBMC) induced by rhC5a and its feature.Method PBMCs were incubated with rhC5a in 5% CO 2 at 37?℃ in regular time interval.Cells were subsequently collected and PKC activity was determined.Result PKC activity in PBMC induced by rhC5a increased with a fashion of double peak.Conclusion The process of intracelluar signal transduction in C5a was associated with the activition of PKC.

参考文献：

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