一氧化氮在L-精氨酸致大鼠残余肾代偿性增生中的作用-东南大学学报医学版

一氧化氮在L-精氨酸致大鼠残余肾代偿性增生中的作用

单位：1.南京铁道医学院附属医院,肾脏科,江苏南京,210009； 2.Sheffield Kidney Institute, University of Sheffield,UK

分类号：R-33, R699.2, O611.62, R977.4

出版年·卷·期（页码）：2000·19·第三期（152-155）

摘要：

目的:探讨一氧化氮(NO)在L-精氨酸(L-arg)所致肾大部切除(SNx)大鼠残余肾代偿性增生中的作用.方法:采用5/6肾大部切除大鼠为实验模型,实验组于手术后分别给予1%L-arg 或NO供体Molsidomine (MSD).实验分为假手术组(Sham)、SNx组、SNx加L-arg组和SNx加MSD组,观察指标为体重(BW)、残余肾重(KW)、肾重/体重(KW/BW)、尿蛋白定量、血压、Ccr、残余肾代偿性增生比率(CRG)、残余肾蛋白质、DNA含量、小管间质细胞PCNA免疫组化表达、尿NO代谢产物NO2-排泄量等.结果:L-arg组大鼠残余肾代偿性增生指标(KW、KW/BW、CRG、蛋白质、DNA及PCNA等)较其对照组明显增加(P小于0.05或0.001),MSD组上述指标与SNx组相比无显著统计学差异(P>0.05).L-arg及MSD组NO2-排泄量均较SNx组显著增加.结论:L-arg可刺激大鼠残余肾代偿性增生,这种作用可能与NO无关.

Objective To investigate the role of nitric oxide (NO) in L arginine ( L arg) potentiated compensatory renal growth in subtotal nephrectomy rats (SNx).Method 5/6 subtotal nephrectomy rats were used as the experimental model.The experimental rats were treated by 1% L arg and NO donor,molsidomine (MSD) after the operation.Four groups were designed in the experiment:Sham,SNx,SNx+ L arg,SNx+MSD.The following parameters were observed:body weight (BW),remnant wet kidney weight (KW),KW/BW,urinary protein excretion,systolic blood pressure,Ccr,compensatory renal growth rate (CRG), protein and DNA content of the remnant kidney,tubulointerstitial immunostaining for PCNA, and excretion rate of NO 2 -.Results SNx rats treated by L arg showed significantly increasing of KW ( P <0.05), KW/BW ( P <0.05), CRG( P <0.05),protein ( <0.05) and DNA content( P <0.001),tubulointerstitial immunostaining for PCNA ( P <0.05),while the group treated by MSD showed no significant changes for the above parameters compared with those in SNx group ( P > 0.05 ,respectively).However,both groups showed the significantly increased urinary excretion of NO 2 -.Conclusion This pilot study suggested that compensatory renal growth potentiated by L arg might not be related with the activation of L arg/NO pathway.

参考文献：

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