正常足月妊娠和妊高征胎盘内皮型一氧化氮合酶免疫荧光分析-东南大学学报医学版

正常足月妊娠和妊高征胎盘内皮型一氧化氮合酶免疫荧光分析

单位：1.南京铁道医学院附属医院,妇产科,江苏,南京,210009； 2.南京铁道医学院附属医院,病理科,江苏,南京,210009

分类号：R714.24, R714.1

出版年·卷·期（页码）：2000·19·第二期（98-100）

摘要：

目的:探讨正常足月妊娠和妊高征胎盘内皮型一氧化氮合酶(NOS3)的分布及其对胎儿胎盘循环的影响.方法:收集正常足月妊娠胎盘10只,妊高征足月胎盘16只(轻度6只,中重度10只),采用间接免疫荧光组织化学法分析NOS3的分布及其含量.结果:正常足月妊娠和妊高征胎盘NOS3的分布范围无明显差异,但后者荧光强度减弱,轻度与中重度之间无差别.结论:足月妊娠胎盘NOS3的分布以滋养层和脐血管内皮为主,其含量的降低可能与参与妊高征的发病过程有关.

Objective The purpose of the work was to study the distribution of NOS 3 in term placenta of normal pregnancy and pregnancy-induced hypertension(PIH) and its effect on the feto-placental circulation.Method The NOS 3 distribution and levels were detected by indirect immunofluorescent method in 26 term placentas including 10 normal term placentas and 16 PIH term placentas.Results A significant difference in distribution of NOS 3 was found neither between normal pregnancy and PIH,nor between mild and severe PIH.A weakened fluorescent light was found in PIH group.Conclusions For term placenta,NOS 3 locates in endothelium of umbilical vessels and syncytiotrophoblast.The decreased concentration of NOS 3 in placenta maybe plays an important role in etiology of PIH.

参考文献：

[1] Friedman S A, Lubansky S L, Ahokas R A. Preeclampsia and related disorders-clinical aspects and relevance of endothelin and nitric oxide, 1995(2)
[2] Dsivid R H, Keith L M, Joseph A L. Vascular smooth muscle:a review of the molecular basis of contractility, 1991
[3] Akar F, Ark M, Uydes B S. Nitric oxide production by human umbilical vessels in severe preeclampsia, 1994(11)
[4] Ghabour M S, Eis A L W, Brockman D E. Immunohistochemical characterization of placental nitric oxide synthase expression in preeclampsia, 1995(9)
[5] 陈敦金, 郑美玉. 胎盘组织一氧化氮合成酶活性的测定及其临床意义, 1996(10)
[6] Koglin J, Glysing-Jensen T, Mudgett J S. Exacerbated transplanted arteriosclerosis in inducible nitric oxide deficient mice. 1998(20)

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录