凋亡相关蛋白BCL-2与白血病化疗药物敏感性的研究-东南大学学报医学版

凋亡相关蛋白BCL-2与白血病化疗药物敏感性的研究

单位：1.南京铁道医学院附属医院,血液科,江苏,南京,210009； 2.上海铁道大学附属甘泉医院-血液科,上海,200065

关键词：白血病 BCL-2蛋白化疗药物敏感性 Western blotting技术四甲基偶氮唑盐比色法

分类号：R733.71, R730.53

出版年·卷·期（页码）：2000·19·第一期（42-44）

摘要：

目的:研究急性白血病(AL)细胞BCL-2表达及其与体外化疗药物敏感性的关系.方法:采用Western blotting技术和四甲基偶氮唑盐比色法(MTT法),分别检测HL-60、U937细胞株和6例急性髓性细胞白血病(AML)细胞BCL-2表达水平及体外对柔红霉素(DNR)的敏感性.结果:(1)BCL-2表达水平HL-60细胞高于U937细胞,而6例AML细胞BCL-2表达水平不同;(2)MTT检测结果显示,DNR对HL-60抑制率低于U937;BCL-2高表达的AML细胞,DNR抑制率低.结论:细胞系和白血病原代细胞的BCL-2高表达伴低药敏与AML疗效有关.

Objective To investigate the relationship between the expression of BCL?2 protein and the chemosensitivity of acute leukemic cells in vitro .Method The levels of BCL?2 protein and susceptibility to DNR(daunorubicin) in HL?60 cells,U 937 cell lines and the bone marrow cells of six cases of AML were detected by the method of Western blotting and MTT assay.Results (1) The HL?60 cells have a higher BCL?2 protein expression than U 937 cells.All of the six cases of AML have different levels of BCL?2 protein;(2) The U 937 cells are more susceptible to DNR than HL?60,while in AML cases,the higher levels of BCL?2 protein were associated with poor susceptibility. Conclusion High levels of BCL?2 protein may associate with poor response to chemical therapy.

参考文献：

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