L-精氨酸对人肾系膜细胞增殖的影响-东南大学学报医学版

L-精氨酸对人肾系膜细胞增殖的影响

单位：1.南京铁道医学院附属医院,肾内科,江苏,南京,210009； 2.南京铁道医学院,医学科学研究所,江苏,南京,210009； 3.南京铁道医学院附属医院,心内科,江苏,南京,210009

分类号：R329.2+8, R322.6+1, R692.6, Q517

出版年·卷·期（页码）：2000·19·第一期（20-22）

摘要：

目的:探讨L-精氨酸对体外培养的人肾系膜细胞(HMCL)增殖的影响.方法:用四甲基偶氮唑盐比色法测定不同浓度L-精氨酸培养液中HMCL的增殖情况.结果:随L-精氨酸浓度增加,细胞生长受抑制.L-精氨酸浓度大于20-mmol.L-1时,细胞生长的抑制与对照组比较有统计学意义.选择L-精氨酸敏感浓度25-mmol.L-1评价其时间效应,0～48-h内抑制率增加.结论:L-精氨酸抑制人肾系膜细胞增殖,呈浓度、时间依赖性,其对治疗慢性肾纤维化具有潜在价值.

Objective The experiment was designed to study the effect of L ?arginine on the proliferation of human mesangial cells in vitro by using 3?(4,5?dimethylthiazole?2?yl)?2,5?diphenyl tetrazolium bromide(MTT) method. Methods The effects of L ?arginine on human mesangial were cells examined by cell culture technology and the method of MTT.Result Cell growth was significantly inhibited when the concentration of L ?arginine in the media was more than 20?mmol·L -1 compared with the control group, which showed dose?dependent manner. Furthermore, the antiproliferative effect of L ?arginine was demonstrated as time?dependent manner as well in this experiment,inhibitive rate increasing in 0～48?h at 25?mmol·L -1 . Conclusion L ?arginine might exert an antiproliferative effect on the human renal mesangial cells with a dose?and time?dependent manner.

参考文献：

[1] Chang C I, Liao J-C, Kuo L. Arginase modulates nitric oxide production in activated macrophages, 1998
[2] Mene P, Simonson M S, Dunn M J. Physiology of the mesangial cell, 1989(4)
[3] Mackay K, Striker L J, Stauffer J W. Transforming growth factor-β.Murine glomerular receptors and responses of isolated glomerular cells, 1989(4)
[4] Kashgarian M, Sterzel R B. The pathobiology of the mesangium. 1992(3). doi:10.1038/ki.1992.74
[5] Floege J, Eng E, Young B A. Factors involved in the regulation of mesangial cell proliferation in vitro and in vivo, 1993(zk)
[6] Bruijn-J A, Hogendoorn-P C W, Hoedemaeker-PJ. The extracellular matrix in pathology, 1988(2)
[7] Blum M, Yachnin T, Wollman Y. Low nitric oxide produc-tion in patients with chronic renal failure. 1998(3). doi:10.1159/000045047
[8] Studer R K, Craven P A, DeRubertis F R. Thromboxane stimulation of mesangial cell fibronectin synthesis is signalled by activation of protein kinase C and is modulated by cGMP, 1993
[9] Trachtman H, Futterweit S, Garg P. Nitric oxide stimulates the activity of a 72-kDa neutral matrix metalloproteinase in cultured rat mesangial cells. 1996. doi:10.1006/bbrc.1996.0125
[10] Reyes A-A, Purkerson M L, Karl I. Dietary supplementation with L-arginine ameliorates the progression of renal diseases in rats with subtotal nephrectomy, 1993(2)

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