逆转录病毒介导IL-2cDNA治疗小鼠淋巴瘤的研究-东南大学学报医学版

逆转录病毒介导IL-2cDNA治疗小鼠淋巴瘤的研究

单位：1.南京铁道医学院,生物学教研室,江苏,南京,210009； 2.中国药科大学,生物化学教研室,江苏,南京,210009； 3.南京铁道医学院,病理学教研室,江苏,南京,210009

分类号：R73-36+2, R730.51, R733

出版年·卷·期（页码）：2000·19·第一期（1-4）

摘要：

目的:研究人白细胞介素-2(hIL-2)转基因小鼠淋巴瘤体内成瘤性.方法:体外将hIL-2基因转入小鼠淋巴瘤p388细胞.用PCR、RT-PCR、dot blot法检测hIL-2基因在细胞内的整合及表达,四甲基偶氮唑盐比色(MTT)法检测转导细胞hIL-2分泌.体内试验将动物分为A9组、对照组和体内转染治疗组3组.17-d后处死所有动物,观察肿瘤生长状况.结果:hIL-2基因已成功地整合到小鼠p388细胞基因组内.p388/IL-2细胞分泌的hIL-2 量为37.4-U.ml-1.A9组和体内转染治疗组动物肿瘤体积明显减小,与对照组肿瘤体积有显著性差异(P<0.05).结论:hIL-2基因经体内或体外法转入p388/IL-2细胞后减弱了在小鼠体内的致瘤性.

Objective The experiment was designed to study the tumorigenesis of human interleukin?2(hIL?2) transgenetic mice lymphoma.Methods The hIL?2 gene was transferred into mice lymphoma cell line in vitro . The DNA and RNA of hIL?2 transduced p388 cell were analyzed by PCR,RT?PCR,dot?blot.The secretion of hIL?2 was measured by 3?(4,5?dimethylthiazole?2?yl)?2,5?diphenyl?tetrazolium bromide(MTT) method.The experimental animals were invided into 3 groups.Group 1 was treated with A9 cells,group 2 served as control and group 3 was treated with p338?1 cells and A8 supernatant.The size and weight of tumor were measured at day 17 after the treatment.Results The hIL2 cDNA had been inserted into the genome of p388/IL?2 cell and had the expression of hIL2 mRNA, p388/IL?2 cell would secret 37.4?U·ml -1 hIL?2. The tumor size in group 1 and 3 reduced markedly and significantly differed from that in control group( P <0.05).Conclusion The tumorigensis of p388/IL2 cell,which was transduced by hIL2 gene in vivo or in vitro ,was reduced in mice.

参考文献：

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