高效液相色谱法测定人血清中头孢他啶的浓度-东南大学学报医学版

高效液相色谱法测定人血清中头孢他啶的浓度

单位：1.东南大学基础医学院病理学与病理生理学系,江苏,南京,210009； 2.西宁铁路医院,妇产科,青海,西宁,810009

分类号：O652.63, R927.2

出版年·卷·期（页码）：2004·23·第六期（388-390）

摘要：

目的:建立高效液相色谱(HPLC)法测定人血清中头孢他啶浓度的方法.方法:HPLC法采用的色谱柱为C18柱,流动相为甲醇-0.02mol·L-1磷酸二氢钾溶液(1.5:8.5),检测波长为254nm.结果:线性范围为0.5～80mg·L-1(r=0.999 25),回收率为81.69%～100.01%,日内RSD为2.96%～5.80%,日间RSD为3.50%～6.96%.结论:HPLC法简便、快速、准确,可用于药代动力学研究以及患者血药浓度的监测.

Objective To set up method of high performance li quid chromatography(H PLC) to determine ceftazidime in human serum. Method HPLC was used,with its coloum of C18, the mobile phase of methanol∶KH 2PO 4(1.5∶8.5), the detector wave of 254-nm. Results The calibration curve was in the range of 0.5～80-mg·L -1. The recovery was 81.69%～100.01%, within- da y and intra-day RSD were 2.96%～5.80% and 3.50%～6.96%. Conclusion The method is simple,rapid and accurate. It was special for the studies of pharmacokinetics and determination the serum conceratration of ceftazidime.

参考文献：

[1] 陈新谦, 金有豫, 汤光. 新编药物学, 2003
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[6] 国家药典委员会. 中国药典(二部), 2000

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