ST-1抗豚鼠和兔心肌缺血再灌注损伤的血流动力学研究-东南大学学报医学版

ST-1抗豚鼠和兔心肌缺血再灌注损伤的血流动力学研究

单位：东南大学基础医学院生理学与药理学系,江苏,南京,210009

分类号：R965, R972

出版年·卷·期（页码）：2004·23·第六期（384-387）

摘要：

目的:拟证实双萜类化合物ST-1的抗心肌缺血再灌注损伤作用.方法:(1)Langendorff装置逆向豚鼠心脏灌注,预先灌注ST-1 1.0、5.0和10μmol·L-15min,停灌30min,再灌注20min,制成豚鼠心肌缺血再灌注损伤模型,观察药物对心脏舒缩功能、冠脉流出液量的影响;(2)结扎麻醉兔冠脉左前降支30min后再连续灌注60min,制成兔心肌缺血再灌注损伤模型,ST-125～100μg·kg -1静脉注射,动态观察其对血流动力学指标的影响.结果:ST-1预处理可有效减轻离体豚鼠缺血再灌注引起的左室舒缩功能下降;兔ST-1组左心室等容期压力最大变化速率、左心室收缩压、左心室发展压、左心室压峰值时间的变化值明显小于模型对照组(P＜0.05),心率、舒张压、收缩压和平均动脉压变化不明显(P＞0.05).结论:ST-1预处理有抗离体豚鼠和在体家兔心肌缺血再灌注损伤的作用.

Objective To study the effects of ST-1,a diterpen e c ompound, on myocardial dysfunction induced by ischemia reperfusion.Meth ods The isolated guinea-pig heart was perfused with modified Tyrde bu ffer solution by Langendorff. ST-1 1,5,10-μmol·L -1 were perfused 10 min prior to ischemia, ischemia 30 min and reperfusion 20 min in vitro,respecti vely. The changes of hemodynamics were observed in rabbits with m yocardial ischemia reperfusion model induced by occluding the left anterior desc ending coronary artery(LAD) for 30 min followed by 60 min reperfusion.R esults The preconditioning with ST-1 could effectively preserve the ca rdiac function.The values of change in LVSP,LVDP’,±dp/dt max,T-dp/dt m a x induced by ST-1 were significantly less than the respective value of vehi c le control inanesthetized and ischemia reperfused rabbits.There were no signifi can t changes of heart rate and blood pressure by administration of ST-1.Co n clusion Preconditioning with ST-1 could ameliorate cardiac dysfunction induced by ischemia and reperfusion significantly.

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