茶多酚对高糖所致人肾小球系膜细胞Ⅳ型胶原合成的影响-东南大学学报医学版

茶多酚对高糖所致人肾小球系膜细胞Ⅳ型胶原合成的影响

单位：1.东南大学基础医学院病理学与病理生理学系,江苏,南京,210009;东南大学,肾病研究所,江苏,南京,210009； 2.东南大学医学院附属徐州医院内分泌科,江苏,徐州,221009； 3.东南大学基础医学院病理学与病理生理学系,江苏,南京,210009； 4.东南大学,肾病研究所,江苏,南京,210009;东

关键词：茶多酚高糖人肾小球系膜细胞 Ⅳ型胶原

分类号：R363.21

出版年·卷·期（页码）：2004·23·第六期（366-369）

摘要：

目的:探讨高糖对人肾小球系膜细胞Ⅳ型胶原合成的影响及茶多酚的干预作用.方法:应用放射免疫法检测高糖条件下体外培养的系膜细胞上清液中Ⅳ型胶原含量及茶多酚干预后其含量的改变;应用免疫细胞化学法检测高糖条件下系膜细胞内Ⅳ型胶原的表达以及茶多酚干预后的变化.结果:高糖可增加系膜细胞上清液及细胞内Ⅳ型胶原的含量;茶多酚可抑制高糖条件下上清液中及细胞内Ⅳ型胶原的增加.结论:高糖可导致人肾小球系膜细胞Ⅳ型胶原合成增加,而茶多酚可有效干预此过程.

Objective To investigate the effect of high gluco se on the level of type Ⅳ collagen in human mesangial cells and the intervention of tea polypheno ls (TPs). Methods The cells were cultured in high glucose with or without TPs. The contents of type Ⅳ collagen in the media were detected by r adioimmunoassay. Immunocytochemistry was used to detect the expression of type Ⅳ collagen in the cells. Results Level of type Ⅳ collagen in human mesangial ce lls increased cul tured with high glucose,however,it decreased co-cultured with TPs compared with that only with high glucose. Conclusion High glucose contributes to an i ncrease of typ e Ⅳ collagen produced by human mesangial cells and TPs can reduce high glucose -induced type Ⅳ collagen.

参考文献：

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