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高糖环境下肾小球细胞间相互作用对TGF-β1表达的影响及茶多酚的干预作用
作者:邓红1 李海浪2 王凯2 王筠3 刘必成4 
单位:1.东南大学基础医学院病理学与病理生理学系,江苏,南京,210009;东南大学,肾病研究所,江苏,南京,210009; 2.东南大学附属中大医院儿科,江苏,南京,210009; 3.东南大学基础医学院病理学与病理生理学系,江苏,南京,210009; 4.东南大学,肾病研究所,江苏,南京,210009;东南大学附属
关键词:细胞间相互作用 肾小球系膜细胞 内皮细胞 高糖 茶多酚 转化生长因子-β1mRNA 
分类号:R587.202
出版年·卷·期(页码):2004·23·第六期(362-366)
摘要:

目的:探讨在高糖环境下人肾小球系膜细胞与内皮细胞间相互作用对TGF-β1mRNA表达的影响和茶多酚的干预作用.方法:建立系膜细胞和内皮细胞共培养模型,分空白对照组、高糖组、茶多酚干预组和茶多酚对照组,培养0、12、36 h后,应用RT-PCR法检测模型中两种细胞转化生长因子-β1(TGF-β1)的表达情况.结果:高糖可上调共培养模型中系膜细胞和内皮细胞TGF-β1mRNA表达,其作用显著强于单独培养的系膜细胞和内皮细胞,茶多酚可降低共培养模型中细胞TGF-β1mRNA表达,且效果优于单独培养.结论:(1)高糖环境下系膜细胞和内皮细胞间存在相互作用,这种作用能促进两种细胞TGF-β1mRNA高表达.(2)茶多酚通过对TGF-β1mRNA表达的抑制影响细胞间相互作用.

Objective  To study the effect of high glucose and tea polyphenols  (TPs) on the interaction of human mesangial cells and endothelial cells.  Methods  Mesangial cells and endothelial cells were cultured alone or  together in high  glucose media with or without TPs for 0-h, 12-h and 36-h respectively. The ex pressi ons of TGF-β  1-mRNA in the cells were measured by RT-PCR. Resul ts  High glucose i ncreased the expression of TGF-β  1-mRNA in co-cultured mesangial cells  as well as  in co-cultured endothelial cells and the effect became more prominent than in  sing le-cultured cells,which could be interruptted by TPs more effectively in co-cu ltured cells than in single-cultured  cells. Conclusions  (1)There is some kind of interaction betwee n human mesangia l cells and endothelial cells in high glucose condition, and the interaction can  increase the expression of TGF-β  1-mRNA. (2)TPs inhibit the expression  of TGF-β  1-mRNA by affecting the intercellular interaction between the  mesangial c ells and the endothelial cells.

参考文献:

[1] ZIYADEH F N. Mediators of diabetic renal disease:the case for TGF-β as the major mediator. 2004(Suppl 1). doi:10.1097/01.ASN.0000093460.24823.5B
[2] Isaka Y, TSUJIE M, ANDO Y. Transforming growth factorbeta 1 antisense oligodexynucleotodes block interstitial fibrosis in unilateral ureteral obstruction. 2000. doi:10.1111/j.1523-1755.2000.00360.x
[3] 曾磊, 张玉军. 茶多酚的功能特性及应用, 2003
[4] 王凯, 邓红, 苏宁. 茶多酚对高糖环境下系膜细胞和内皮细胞在活性氧方面相互作用的影响, 2004
[5] 王蓓, 李凯, 邓红. 茶多酚对糖尿病肾病大鼠肾脏转化生长因子表达的影响. 中日友好医院学报2003(5). doi:10.3969/j.issn.1001-0025.2003.05.010
[6] 王蓓, 邓红, 苏宁. 茶多酚对糖尿病大鼠活性氧表达的影响. 东南大学学报(医学版)2002(1). doi:10.3969/j.issn.1671-6264.2002.01.010 

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