人瘢痕成纤维细胞中Sp-1等转录因子的研究-东南大学学报医学版

人瘢痕成纤维细胞中Sp-1等转录因子的研究

单位：1.东南大学附属中大医院整形外科,江苏,南京,210009； 2.上海第二军医大学附属长征医院,整形外科,上海,200003

分类号：Q813, R364.3

出版年·卷·期（页码）：2004·23·第六期（359-361）

摘要：

目的:研究增生性瘢痕成纤维细胞中纤维化相关的转录因子.方法:人皮肤增生性瘢痕成纤维细胞原代、传代培养后抽提核蛋白.人工法合成转录因子Sp-1、NF-Ⅰ、NF-кB识别序列的双链寡核苷酸,并采用Dig-ddUTP3'末端标记该双链寡核苷酸.电泳迁移率改变实验(EMSA)法研究瘢痕成纤维细胞中纤维化相关的Sp-1、NF-Ⅰ、NF-кB等转录因子.结果:在人增生性瘢痕成纤维细胞的核蛋白中存有纤维化相关的Sp-1、NF-Ⅰ、NF-κB等转录因子.结论:瘢痕纤维化的发生、发展与纤维化相关的转录因子SSp-1、NF-Ⅰ、NF-кB密切相关.

Objective To analyze the transcription factors r elated to fibrosis in human hypertrophic scar fibroblasts. Methods Fibroblasts ob tained from the hypertrophic scar of a 3-year-old patient were primarily cultured and subcultu red in vitro, and then nucleoproteins were extracted from the cells. The double-st randed oligonucleotides specifically recognized by transcription factors Sp-1 , NF-Ⅰ, NF-κB were synthesized and end-labeled with Dig-ddUTP, then used a s a pr obe in electrophoretic mobility shift assays(EMSA) to identify the transcription factors related to fibrosis in hypertrophic scar fibroblasts. Result The data confirmed that there were transcription factors Sp-1, NF-Ⅰ, NF-κB in the hype rtrophic scar fibroblasts. Conclusion Hypertrophic scar develop ing is related to transcription factors Sp-1, NF-Ⅰ, NF-κB.

参考文献：

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