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| 氨磷汀对顺铂和环磷酰胺诱导正常细胞毒性的保护作用 |
| 作者：韩国荣1 叶银英2 余敏敏1 何道伟2 |
| 单位：1.东南大学医学院,附属南京第二医院,江苏,南京,210003； 2.东南大学医学院,现代医学实验中心,江苏,南京,210009 |
| 关键词：氨磷汀 顺铂 环磷酰胺 化学治疗 细胞毒性 骨髓有核细胞微核率 小鼠 |
| 分类号：R453, R-33 |
| 出版年·卷·期（页码）：2004·23·第五期（314-316） |
| 摘要： |
目的:评价氨磷汀对顺铂和环磷酰胺诱导正常细胞毒性的保护作用及对化疗药物抗肿瘤作用的影响.方法:用MTT法测定顺铂对体外培养的Hela、Ho-8910肿瘤细胞的抑制作用,实验组加顺铂前30 min经氨磷汀处理,对照组则不需处理,比较两组细胞增殖的抑瘤率.同时观察注射顺铂、环磷酰胺前30 min注射氨磷汀与不注射组小鼠骨髓有核细胞的微核率.结果:用600 mg·L-1氨磷汀在体外加顺铂前30 min加入Hela、Ho-8910肿瘤细胞,和对照组比较两组的肿瘤细胞增殖没有差异(P>0.05);而体内实验得到,顺铂、环磷酰胺腹腔注射正常小鼠前30 min注射氨磷汀与不注射氨磷汀比较,单独用顺铂、环磷酰胺的小鼠骨髓有核红细胞微核率高于氨磷汀保护组(P<0.05).结论:氨磷汀腹腔注射正常小鼠可明显降低顺铂、环磷酰胺引起正常小鼠的细胞毒性,同时氨磷汀不影响顺铂对Hela、Ho-8910肿瘤细胞的化疗疗效. |
Objective To evaluated the protective ability of amifostine on cytotoxicities due to chemotherapy without affecting its tumorcidal effect. Methods Two cell lines(Hela,Ho-8910) were exposed to cisplatine. Cell were pretreated with either 0 or 600、mg·L -1 amifostine. Tumorcidal effect in two groups was measured using the MTT assay.The mice were pretreated with amifostine 30 minutes before each cisplatine and cyclophosphamide in the test group, while mice in control group were not pretreated with amifostine. The micronuclei of mice was observed in two groups. Results There was no significant difference in antitumor effect between drug-treated samples with and without pretreatment with amifostine(P>0.05). The incidence rate of micronuclei of mice in control group were significantly higher than that of the test group(P<0.05). Conclusion Amifostine protects against a spectrum of cytotoxicities in mice induced by cisplatine and cyclophosphamide it without affecting tumorcidal effect. |
| 参考文献： |
[1] 马培奇. 细胞保护剂氨磷汀及其临床应用及展望. 中国肿瘤2001(8). doi:10.3969/j.issn.1004-0242.2001.08.015 |
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