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黄芪对新生大鼠脑缺氧缺血损伤后海马治疗作用的研究
作者:蒋犁1 贾瑞喆1 乔立兴1 陈平圣2 
单位:1.东南大学,临床医学院,江苏,南京,210009; 2.东南大学基础医学院,病理学与病理生理学系,江苏,南京,210009
关键词:脑缺氧 脑缺血 半胱氨酸蛋白酶 细胞凋亡 黄芪 大鼠 
分类号:R-332, R722.1, R282.71
出版年·卷·期(页码):2004·23·第五期(291-294)
摘要:

目的:探讨新生儿缺氧缺血脑损伤(hypoxia-ischemia brain damage,HIBD)后,脑细胞损伤的机制和黄芪对海马区的神经保护作用.方法:用新生大鼠建立新生儿HIBD模型,于缺氧后不同时间点取脑,分别行组织病理学检查并计数海马CA1区神经细胞死亡率、免疫组化检测结扎侧海马区天冬氨酸特异酶切的半胱氨酸蛋白酶-3(caspase-3)蛋白的表达,三等分迷宫测试成熟大鼠学习记忆能力.分假手术组、模型组和黄芪治疗组观察并对比上述指标.结果:模型组结扎侧海马caspase-3蛋白表达于缺氧缺血(HI)后6 h轻度升高,24 h达高峰,48 h后下降,5 d和7 d时渐恢复至基础水平.黄芪组HI后结扎侧海马神经细胞死亡率明显降低、caspase-3蛋白的表达峰值降低了35%~41%,成熟大鼠的学习记忆能力明显提高.结论:黄芪对未成熟脑HIBD后海马部位有明显的神经保护功能,此功能与抑制caspase-3的表达有关.

Objective  To study on the mechanism of neuron death due to hypoxia-ischemia brain damage (HIBD) and the neuroprotective effects of astragulus membraneaceus on neonatal cerebral hippocampus of HIBD. Methods  The neonatal hypoxia-ischemia model was established in rat pups of 7-day-old. Brain injury was examined by neuron death rate in the cerebral hippocampus CA1. Immunohistochemical staining with cysteinyl aspartate-specific proteinase (caspase-3) antibody was used to detect the changes in caspase-3 protein expression in ipsilateral hippocampus. 90 days old rats was used in tri-equal-arm maze to observe discrimination learning ability. Sham,model and astragulus membraneaceus treated groups were set up. Results  In model group, caspase-3 protein showed an increase at 6、h, reaching a maximum at 24~48、h after hypoxia-ischemia. In astragulus-membraneaceus treated group, neurons death rate、caspase-3 protein were significantly reduced by astragulus membraneaceus, and discrimination learning ability of developed rats were improved obviously. Conclusion  Astragulus membraneaceus produces a strong protective effect against neuronal damage in the immature rat hippocampus, along with a reduce in caspase-3 expression.

参考文献:

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