Objective The purpose of this study is to optimize the different parameters in the expression of recombinant human placental anticoagulant protein derivative expressing E.coli strain.Method The relationships between plasmid stability,bacterial growth and density,the dosage of expression inducer IPTG,the induction duration and the expression level of recombinant protein were extensively investigated.Results The recombinant human placental anticoagulant protein derivative expressing E.coli strain maintained very good plasmid stability and the plasmid loss did not occur.The more the IPTG inducer,the lower the bacterial density at OD 600.Longer induction resulted in more accumulation of target proteins,and the bacterial density at OD 600 was proportionate to the total cellular proteins.Conclusion After optimizing the expression of recombinant human placental anticoagulant protein derivative based upon the above explorations,an overall yield of 40?mg purified recombinant human placental anticoagulant protein derivative protein/liter flask culture was obtained. |
