一个新的基因转录本的克隆及序列分析-东南大学学报医学版

一个新的基因转录本的克隆及序列分析

单位：南京医科大学生殖医学实验室,江苏,南京,210029

分类号：Q781, R394

出版年·卷·期（页码）：2004·23·第四期（214-216）

摘要：

目的:筛选与精子发生和(或)睾丸发育相关的基因.方法:将不同发育阶段(胚胎和成人)的睾丸组织cDNA探针与人睾丸cDNA芯片进行杂交,通过杂交信号的比较,筛选出差异表达的基因.结果与结论:经测序发现该基因全长1 316 bp,包含1个1 056 bp的开放阅读框,编码351个氨基酸.生物信息学序列分析表明,它是p44S10基因家族的一条新的转录本基因,该基因的氨基酸序列编码一26S蛋白酶体调控亚基,在进化中高度保守,提示了该基因在生物体中的重要作用.

Objective To identify genes involved in spermatogenesis and testicular development.Methods Hybridization of human adult and fetal testes cDNA samples was performed with a human cDNA microarray.Results and Conclusions By analysing the hybridization signals,a gene with a differential expression in adult and fetal testis was identified.The full length cDNA of 1?316?bp contained an open reading frame of 1?056?bp which encoded a putative protein of 351 amino acids.Further sequence analysis showed that it was a splicing variant of p44S10 gene family. The deduced protein encoding a regulatory particle of 26S proteasome has been highly conserved during evolution,suggesting the importance of this gene transcript in organism.

参考文献：

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