卡介苗有效成分罗莫肽刺激人PBMCs表达IL-12、IL-4和GM-CSF的研究-东南大学学报医学版

卡介苗有效成分罗莫肽刺激人PBMCs表达IL-12、IL-4和GM-CSF的研究

单位：1.东南大学附属中大医院,泌尿外科,江苏,南京,210009;东南大学基础医学院,病原生物学与免疫学系,江苏,南京,210009； 2.东南大学基础医学院,病原生物学与免疫学系,江苏,南京,210009； 3.东南大学附属中大医院,泌尿外科,江苏,南京,210009

关键词：卡介苗罗莫肽人外周血单个核细胞细胞因子

分类号：R392.1

出版年·卷·期（页码）：2004·23·第三期（169-172）

摘要：

目的:探讨卡介苗(BCG)细胞壁有效成分罗莫肽(RM)对人外周血单个核细胞(HPBMCs)表达细胞因子的刺激作用.方法:获得正常人HPBMCs,采用不同浓度的RM体外刺激HPBMCs72h,收集培养上清液,用ELISA法检测上清液中IL-4、IL-12和GM-CSF的含量.结果:RM能诱导HPBMCs产生较高水平的IL-12和GM-CSF,并呈剂量依赖性;但对IL-4的产生没有促进或抑制作用.结论:RM能有效诱导人外周血单个核细胞产生诱导细胞免疫应答的细胞因子IL-12和GM-CSF,为下一步探讨RM治疗膀胱肿瘤的可行性以及进一步研究其增强策略提供先期的实验基础.

Objective To investigate the efficacy of romurtide(RM),the effective component of Bacillus Calmette?Guerin (BCG) in cytokine production by human peripheral blood mononuclear cells (HPBMCs).Methods Different concentrations of romurtide were administered to HPBMCs cultured in vitro for 72 hours,from which the cultured supernate was harvested,and the level of IL?12,IL?4 and GM?CSF were detected by ELISA.Results RM could significantly stimulate HPBMCs to produce both IL?12 and GM?CSF,Th1?associated cytokines in a dose-dependent manner.However,RM had no obvious effect on the secretion of IL?4,the Th2?associated cytokine.Conclusion RM might be an effective adjuvant to induce cellular immune response via the secreation of IL?12 and GM?CSF of human peripheral blood mononuclear cells,suggesting that romurtide might be used for bladder cancer immunotherapy.

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