耐药逆转剂对耐药细胞内钙离子浓度的影响-东南大学学报医学版

耐药逆转剂对耐药细胞内钙离子浓度的影响

单位：东南大学医学院附属中大医院血液科,江苏,南京,210009

分类号：R979.1, R915, R329.2

出版年·卷·期（页码）：2004·23·第三期（148-151）

摘要：

目的:探讨耐药逆转剂对耐药细胞K562/A02内游离钙离子([Ca2+]i)浓度的影响.方法:用Fura-2/AM方法测定耐药细胞株K562/A02及其敏感株K562的静息[Ca2+]i水平,并观察耐药调节剂汉防己甲素(Tet)、屈洛昔芬(Drol)单独或联合应用后细胞内[Ca2+]i浓度的变化.结果:静息状态下K562/A02细胞的[Ca2+]i浓度显著高于K562细胞.1μmol*L-1Tet或5μmol*L-1 Drol单独作用于K562/A02细胞引起[Ca2+]i浓度的明显升高,两者联合应用有拮抗作用.结论:K562/A02细胞内[Ca2+]i浓度的增高可能是导致其耐药的原因之一,但耐药调节剂Tet、Drol对耐药细胞[Ca2+]i的影响在其逆转耐药中的作用有待进一步研究.

Objective To study the influence of resistance modifying agents upon i of K562/A02 cells.Methods The variations of i of K562 cells and K562/A02 cells after the treatment of Tet and Drol alone or in combination were detected by using Fura-2/AM.Results (1)The concentration of i in K562/A02 cells was higher than that in K562 cells.(2)1?μmol·L -1 Tet and 5?μmol·L -1 Drol alone increased the i of K562/A02 cells time-dependently and they antagonized each other when used simultaneously.Conclusion Therefore, high i may be one reason resulting multiple drug resistances of K562/A02 cells’ MDR,but the role of resistance modifying agents(RMAs) reversing MDR needs further researches.

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