微卫星不稳定在胃癌中的发生及其与胃癌临床病理特点的关系-东南大学学报医学版

微卫星不稳定在胃癌中的发生及其与胃癌临床病理特点的关系

单位：东南大学附属中大医院,消化内科,江苏,南京,210009

分类号：R735.2

出版年·卷·期（页码）：2004·23·第二期（96-99）

摘要：

目的:研究微卫星DNA不稳定性(MSI)在胃癌发生中的作用及其与胃癌临床病理之间的关系.方法:采用PCR、PCR-变性聚丙烯酰胺凝胶及硝酸银染色方法检测30例胃癌组织的MSI.结果:胃癌MSI阳性率为36.67%(11/30),MSI与胃癌患者的年龄、性别、胃癌分期、淋巴结转移及组织分型无关(P＞0.05),与胃癌的分化程度和发生部位有关:高-中分化胃癌high-level MSI(MSI-H)检出率(47.06%,8/17)显著高于低分化胃癌(7.69%,1/13,P＜0.05);胃窦胃癌MSI-H阳性率(47.37%,9/19)显著高于胃体及胃底胃癌(0,0/11,P=0.01).结论:MSI可能在胃癌的发生中起一定作用,其与胃癌临床病理特点的关系尚需进一步探讨.

Objective To investigate the role of microsatellite instability(MSI) in carcinogenesis of gastric cancer(GC)and its relationship with clinicopathological features of GC.Methods MSI was examined in 30 surgically resected GC specimens using PCR,PCR denatured polyacrylamide gel based methods.Results MSI was detected in 36.67%(11/30) of GC.No significant correlation was found between MSI status and age,sex,clinical stage,lymph node metastasis and histological types of GC( P >0.05),but there seems a correlation between MSI and differentiation or location of GC.The incidence of MSI?H(high level MSI) in well or moderately differentiated GC(8/17, 47.06% ) was significantly higher than that in poorly differentiated GC(1/13,7.69%)( P <0.05),and MSI?H occurred more frequently in GC located in antrum(9/19,47.37%)than that in body and fundus(0/11,0%)( P = 0.01 ).Conclusion MSI may play a certain role in gastric carcinogenesis,and the relationship between MSI and clinicopathological features of GC needs further investigation.

参考文献：

[1] Shibata D, PEINADO M A, IONOV Y. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. 1994. doi:10.1038/ng0394-273
[2] Wooster R, CLETON-JANSEN A M, COLLINS N. Instability of short tandem repeats (microsatellite) in human cancers. 1994(6). doi:10.1038/ng0294-152
[3] Boland C R, THIBODEAU S N, HAMILTON S R. A national cancer institute workshop on microsatellite instability for cancer detection and familial predisposition:development of international criteria for the determination of microsatellite instability in coloreetal cancer. 1998(22)
[4] WAI K L, JAE J K, JONG G K. Microsatellite instability in gastric intestinal metaplasia in patients with and without gastric cancer. 2000(2)
[5] ANTONIA R S, ANA C S, YOSHIO Y. Marked differences in the frequency of microsatellite instability in gastric cancer from different countries. 1999(10). doi:10.1111/j.1572-0241.1999.01453.x
[6] 周晓东, 房殿春, 罗元辉. 胃癌及肠化组织微卫星不稳定性. 中华病理学杂志1998(5)
[7] de Manzoni G, TOMEZZOLI A, DI-LEO A. Clinical significance of mutator phenotype and chromosome 17p and 18q allelic loss in gastric cancer. 2001(3). doi:10.1046/j.1365-2168.2001.01667.x
[8] WU M S, LEE C W, Shun C T. Distinct clinicopathologic and genetic profiles in sporadic gastric cancer with different mutator phenotypes. 2000(4). doi:10.1002/(SICI)1098-2264(200004)27:4&lt, 403::AID-GCC10&gt, 3.0.CO, 2-1
[9] WIRTZ H C, MULLER W, NOGUCHI T. Prognostic value and clinicopathological profile of microsatellite instability in gastric cancer. 1998(7)
[10] 王永信, 柯杨, 宁涛. 中国人胃癌组织微卫星DNA的不稳定性研究. 中华医学遗传学杂志1998(3)

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录